Open Access

Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells

  • Authors:
    • Seo-Hyeon Song
    • In-Hye Kim
    • Taek-Jeong Nam
  • View Affiliations

  • Published online on: February 1, 2012     https://doi.org/10.3892/ijmm.2012.899
  • Pages: 741-746
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Abstract

Chlorella vulgaris, a unicellular microalgae, exerts various biological effects; however their effect on proliferation signaling pathways in normal cells has not been studied. We investigated the effect of hot water extracts of Chlorella vulgaris (CVE) on cell proliferation and related signaling pathways in rat intestinal epithelial cells (IEC-6). CVE increased the expression of insulin-like growth factor-I receptor (IGF-IR) and the phosphorylation of focal adhesion kinase (FAK) and Src. In addition, CVE induced activation of the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt pathways. We verified the increased phosphorylation of extracellular-signal-related kinase (ERK) and Akt and the increased expression of the PI3K regulatory subunit p85. CVE also influenced the canonical Wnt pathway through increased expression of the nuclear β-catenin, cyclin D1. Tyr-397 of FAK mediates interactions with Src homology 2 (SH2) domains in a number of other signaling proteins, including PI3K, PLC-γ, Shc, Grb7, Src and Nck2. Because CVE induced FAK activation, FAK may affect the Wnt pathway. Addition of a FAK inhibitor decreased the expression of nuclear β-catenin, cyclin D1 and c-myc, and increased the expression of cytosolic β-catenin. We conclude that CVE stimulated proliferation of IEC-6 cells via the MAPK, PI3K/Akt and canonical Wnt pathways, and that this affected the canonical Wnt pathway.

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May 2012
Volume 29 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Song S, Kim I and Nam T: Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells. Int J Mol Med 29: 741-746, 2012
APA
Song, S., Kim, I., & Nam, T. (2012). Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells. International Journal of Molecular Medicine, 29, 741-746. https://doi.org/10.3892/ijmm.2012.899
MLA
Song, S., Kim, I., Nam, T."Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells". International Journal of Molecular Medicine 29.5 (2012): 741-746.
Chicago
Song, S., Kim, I., Nam, T."Effect of a hot water extract of Chlorella vulgaris on proliferation of IEC-6 cells". International Journal of Molecular Medicine 29, no. 5 (2012): 741-746. https://doi.org/10.3892/ijmm.2012.899