7,8-Dihydroxyflavone exhibits anti-inflammatory properties by downregulating the NF-κB and MAPK signaling pathways in lipopolysaccharide-treated RAW264.7 cells

  • Authors:
    • Hye Young Park
    • Gi-Young Kim
    • Jin Won Hyun
    • Hye   Jin Hwang
    • Nam  Deuk Kim
    • Byung-Woo  Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: March 13, 2012     https://doi.org/10.3892/ijmm.2012.935
  • Pages: 1146-1152
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Abstract

7,8-Dihydroxyflavone (7,8-DHF), a member of the flavonoid family, has received considerable attention as a selective tyrosine kinase receptor B agonist. Several studies have indicated that 7,8-DHF has neurotrophic and antioxidant activities. However, little is known about the cellular and molecular mechanisms underlying the anti-inflammatory activity of 7,8-DHF. Therefore, we investigated whether 7,8-DHF affects the expression of inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Our results indicated that 7,8-DHF significantly attenuated secretion of LPS-induced inflammatory mediators nitric oxide (NO), prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) in RAW264.7 cells. Additionally, LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2 and IL-1β was decreased by pre-treatment with 7,8-DHF. Our results also showed that 7,8-DHF reduces LPS-induced nuclear factor-κB (NF-κB) activity via the suppression of the nuclear translocation of NF-κB p65 and the degradation of inhibitor κB (lκB). In addition, 7,8-DHF inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular-signal-related kinase (ERK), p38, and c-Jun N-terminal kinase (JNK). These results suggest that the anti-inflammatory property of 7,8-DHF is related to the downregulation of iNOS, COX-2 and IL-1β, due to NF-κB inhibition as well as to the negative regulation of MAPK activation in RAW264.7 cells. Thus, 7,8-DHF may be a novel therapeutic agent for the prevention of various inflammatory diseases.

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June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park HY, Kim G, Hyun JW, Hwang HJ, Kim ND, Kim B and Choi YH: 7,8-Dihydroxyflavone exhibits anti-inflammatory properties by downregulating the NF-κB and MAPK signaling pathways in lipopolysaccharide-treated RAW264.7 cells. Int J Mol Med 29: 1146-1152, 2012
APA
Park, H.Y., Kim, G., Hyun, J.W., Hwang, H.J., Kim, N.D., Kim, B., & Choi, Y.H. (2012). 7,8-Dihydroxyflavone exhibits anti-inflammatory properties by downregulating the NF-κB and MAPK signaling pathways in lipopolysaccharide-treated RAW264.7 cells. International Journal of Molecular Medicine, 29, 1146-1152. https://doi.org/10.3892/ijmm.2012.935
MLA
Park, H. Y., Kim, G., Hyun, J. W., Hwang, H. J., Kim, N. D., Kim, B., Choi, Y. H."7,8-Dihydroxyflavone exhibits anti-inflammatory properties by downregulating the NF-κB and MAPK signaling pathways in lipopolysaccharide-treated RAW264.7 cells". International Journal of Molecular Medicine 29.6 (2012): 1146-1152.
Chicago
Park, H. Y., Kim, G., Hyun, J. W., Hwang, H. J., Kim, N. D., Kim, B., Choi, Y. H."7,8-Dihydroxyflavone exhibits anti-inflammatory properties by downregulating the NF-κB and MAPK signaling pathways in lipopolysaccharide-treated RAW264.7 cells". International Journal of Molecular Medicine 29, no. 6 (2012): 1146-1152. https://doi.org/10.3892/ijmm.2012.935