Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity.

  • Authors:
    • H Trachtman
    • S Futterweit
    • E Koss
    • M Bogart
    • A Mermelstein
  • View Affiliations

  • Published online on: March 1, 1999     https://doi.org/10.3892/ijmm.3.3.323
  • Pages: 323-330
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Abstract

Biochemical modification of extracellular matrix (ECM) proteins can alter the function in overlying cells. We tested the hypothesis that metal-catalyzed oxidation of native ECM and individual matrix proteins modulates the activity of inducible nitric oxide synthase (iNOS) in cultured rat mesangial cells (RMC). Oxidized modification of native ECM resulted in a 32% increase in iNOS activity (P<0.01) without influencing the response to supplemental L-arginine or to the addition of the iNOS inhibitor, L-NAME. Immunoblot analysis indicated that enhanced iNOS activity was not associated with a parallel rise in the cytosolic content of iNOS. Synthesis of type IV collagen was unaffected by growth of RMC on oxidized native ECM. Oxidation of three normal constituents of the mesangial matrix - type IV collagen, laminin, and fibronectin - also stimulated iNOS activity in overlying RMC by 18-32% (P<0.05). Growth of RMC on oxidized type I collagen or Vitrogel had no effect on NO production. We conclude that oxidized modification of the mesangial matrix promotes increased iNOS activity and NO production by mesangial cells. Further work is required to determine whether this response limits glomerular injury or promotes damage to the mesangium in oxygen free radical-mediated diseases such as chronic renal failure, atherosclerosis and diabetes.

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Mar 1999
Volume 3 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Trachtman H, Futterweit S, Koss E, Bogart M and Mermelstein A: Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity.. Int J Mol Med 3: 323-330, 1999
APA
Trachtman, H., Futterweit, S., Koss, E., Bogart, M., & Mermelstein, A. (1999). Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity.. International Journal of Molecular Medicine, 3, 323-330. https://doi.org/10.3892/ijmm.3.3.323
MLA
Trachtman, H., Futterweit, S., Koss, E., Bogart, M., Mermelstein, A."Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity.". International Journal of Molecular Medicine 3.3 (1999): 323-330.
Chicago
Trachtman, H., Futterweit, S., Koss, E., Bogart, M., Mermelstein, A."Growth of rat mesangial cells on oxidized extracellular matrix increases inducible nitric oxide synthase activity.". International Journal of Molecular Medicine 3, no. 3 (1999): 323-330. https://doi.org/10.3892/ijmm.3.3.323