Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells

  • Authors:
    • Kozue Kobayashi
    • Masumi Tanaka
    • Satoru Nebuya
    • Kenichi Kokubo
    • Yutaka Fukuoka
    • Yoshiteru Harada
    • Hirouske Kobayashi
    • Makoto Noshiro
    • Hidenori Inaoka
  • View Affiliations

  • Published online on: June 11, 2012     https://doi.org/10.3892/ijmm.2012.1023
  • Pages: 509-513
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Abstract

The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to cyclic stretching. IL-6 protein was increased even in cells without stretching. Fold changes determined by dividing the level of IL-6 protein in stretched cells by that in unstretched cells at the same sampling times indicated that IL-6 protein was increased by stretching. At least 1 h of stretching was necessary to elicit an increase of IL-6 protein, and the levels peaked at 3 h after the start of stretching. After withdrawal of stretching, there was no further increase of IL-6 protein. The expression levels of the IL-6 gene were significantly increased by stretching and peaked at 30 min after the start of stretching. The difference in the peak times of IL-6 gene and protein expression likely reflects the process of protein synthesis after the appearance of IL-6 mRNA.
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September 2012
Volume 30 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kobayashi K, Tanaka M, Nebuya S, Kokubo K, Fukuoka Y, Harada Y, Kobayashi H, Noshiro M and Inaoka H: Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells. Int J Mol Med 30: 509-513, 2012
APA
Kobayashi, K., Tanaka, M., Nebuya, S., Kokubo, K., Fukuoka, Y., Harada, Y. ... Inaoka, H. (2012). Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells. International Journal of Molecular Medicine, 30, 509-513. https://doi.org/10.3892/ijmm.2012.1023
MLA
Kobayashi, K., Tanaka, M., Nebuya, S., Kokubo, K., Fukuoka, Y., Harada, Y., Kobayashi, H., Noshiro, M., Inaoka, H."Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells". International Journal of Molecular Medicine 30.3 (2012): 509-513.
Chicago
Kobayashi, K., Tanaka, M., Nebuya, S., Kokubo, K., Fukuoka, Y., Harada, Y., Kobayashi, H., Noshiro, M., Inaoka, H."Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells". International Journal of Molecular Medicine 30, no. 3 (2012): 509-513. https://doi.org/10.3892/ijmm.2012.1023