Open Access

ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer

  • Authors:
    • David Adler
    • Jacqueline Ochsenfahrt
    • Kerstin Fuchs
    • Glen Kristiansen
    • Sven Perner
    • Nicolas Wernert
  • View Affiliations

  • Published online on: August 20, 2012     https://doi.org/10.3892/ijmm.2012.1097
  • Pages: 1029-1033
  • Copyright: © Adler et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The erythroblast transformation-specific (ETS) family of transcription factors plays important roles in both physiological and pathological conditions. Even though many studies have focused on single ETS factors within a single tissue and within the context of specific promoters, the functional impact of multiple ETS members present within a specific cell type has not yet been investigated, especially in prostate cancer (PCa). As the most prominent gene rearrangement in PCa leads to the overexpression of the ETS-related gene (ERG), the aim of this study was to investigate whether ERG is part of a complex integrated transcriptional network that involves other ETS factors. More specifically, as the ETS family consists of 27 members, we focused our efforts initially on investigating whether ERG is associated with the three family members, ETS-1, ETS-2 and ETS variant gene‑4 (ETV‑4), in PCa as a proof of principle. Using western blot analysis, we show that ERG, ETS-1, ETS-2 and ETV-4 are expressed in PC3 cell nuclear extracts and in protein lysates prepared from human PCa prostatectomy specimens. Immunoprecipitations using an anti-ERG antibody were used with PC3 cell nuclear extracts as well as with a pooled protein lysate sample prepared from the PCa tissue samples of five patients. Importantly, our results revealed that ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in PC3 cell nuclear extracts and PCa tissue protein lysates. Our findings strongly support the notion that ERG is part of a complex integrated transcriptional network that involves other ETS factors, which are likely to cooperate or influence the activity of ERG in PCa. The functional impact of multiple ETS factors being associated with ERG in PCa requires further study, as it may provide insights into the mechanism by which ERG exerts its influence in PCa and may subsequently contribute to our understanding of the molecular basis of PCa.
View Figures
View References

Related Articles

Journal Cover

November 2012
Volume 30 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Adler D, Ochsenfahrt J, Fuchs K, Kristiansen G, Perner S and Wernert N: ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. Int J Mol Med 30: 1029-1033, 2012
APA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., & Wernert, N. (2012). ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. International Journal of Molecular Medicine, 30, 1029-1033. https://doi.org/10.3892/ijmm.2012.1097
MLA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30.5 (2012): 1029-1033.
Chicago
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30, no. 5 (2012): 1029-1033. https://doi.org/10.3892/ijmm.2012.1097