Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation

  • Authors:
    • Yaping Ye
    • Weihua Hu
    • Fengjing Guo
    • Weikai Zhang
    • Jiang Wang
    • Anmin Chen
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  • Published online on: August 9, 2012     https://doi.org/10.3892/ijmm.2012.1091
  • Pages: 1075-1080
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Abstract

Biglycan (BGN) has been reported to promote bone morphogenetic protein-4 (BMP-4) stimulated osteoblastic differentiation. However, the underlying mechanism has yet to be fully elucidated. The glycosaminoglycan (GAG) chains of BGN have a variety of biological functions. In the present study, we explored the potential role of the GAG chains of BGN in promoting BMP-4-induced osteoblast differentiation. BGN knockout (KO) murine calvarial cells were transfected with adenovirus overexpressing wild-type BGN (Adv-BGN), adenovirus expressing GAG-mutant BGN (Adv-BGNm) and adenovirus without BGN (Adv-Emp). Transfected cells were treated with or without BMP-4. Subsequently, BMP-4 signaling and function were assessed by evaluating the expression of the osteoblast differentiation-related proteins, Smad1/5/8 phosphorylation and alkaline phosphatase (ALP) activity. Furthermore, the binding specificity of the transfected cells to BMP-4 was also investigated using immunofluorescence staining. Our study demonstrated that a mutant BGN lacking GAG chains decreased BGN-assisted BMP-4 signaling and osteoblast differentiation and that the expression of this mutant BGN in biglycan knockout (BGN‑KO) calvarial osteoblasts could not rescue its differentiation deficiency as efficiently as wild-type (WT) BGN. These results strongly suggest that the GAG chains of BGN promote BGN-assisted BMP-4 function.
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November 2012
Volume 30 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ye Y, Hu W, Guo F, Zhang W, Wang J and Chen A: Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation. Int J Mol Med 30: 1075-1080, 2012
APA
Ye, Y., Hu, W., Guo, F., Zhang, W., Wang, J., & Chen, A. (2012). Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation. International Journal of Molecular Medicine, 30, 1075-1080. https://doi.org/10.3892/ijmm.2012.1091
MLA
Ye, Y., Hu, W., Guo, F., Zhang, W., Wang, J., Chen, A."Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation". International Journal of Molecular Medicine 30.5 (2012): 1075-1080.
Chicago
Ye, Y., Hu, W., Guo, F., Zhang, W., Wang, J., Chen, A."Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation". International Journal of Molecular Medicine 30, no. 5 (2012): 1075-1080. https://doi.org/10.3892/ijmm.2012.1091