Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity

  • Authors:
    • Min-Ho Jeong
    • Kwang-Mo Yang
    • Jung-Ki Kim
    • Byung-Hyouk Nam
    • Gi‑Yong Kim
    • Sang-Wha Lee
    • Su-Yeong Seo
    • Wol-Soon Jo
  • View Affiliations

  • Published online on: November 14, 2012     https://doi.org/10.3892/ijmm.2012.1181
  • Pages: 205-212
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The control of melanogenesis is an important strategy in the treatment of abnormal skin pigmentation for cosmetic purposes. The aim of the present study was to investigate the anti-melanogenic effect of Asterina pectinifera (A. pectinifera) extracts by cell-free mushroom tyrosinase assay, cellular tyrosinase assay, melanin content assay and the analysis of related protein expression in melan-a cells. A. pectinifera was extracted with 80% methanol (80-MAP) and further fractionated with hexane (He-AP) and ethyl acetate (EA-AP). In addition, the enzyme extract (En-AP) of A. pectinifera, to which protease was added, was processed. EA-AP and En-AP among A. pectinifera extracts showed strong inhibitory activity against the cell-free mushroom tyrosinase activity. EA-AP and En-AP induced significant inhibition of melanin production and cellular tyrosinase activity. In the action of EA-AP and En-AP on melanogenesis, they reduced the expression of melanogenic genes and proteins including tyrosinase, tyrosinase-related protein-1 (TRP-1) and dopachrome tautomerase (Dct). These results showed that EA-AP and En-AP inhibited melanogenesis by reducing tyrosinase activity and melanin production via subsequent downregulation of tyrosinase-related proteins. The overall results suggest that EA-AP and En-AP among A. pectinifera extracts may be promising candidates for the treatment of hyperpigmentation disorder and useful for self-tanning cosmetic products.
View Figures
View References

Related Articles

Journal Cover

January 2013
Volume 31 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jeong M, Yang K, Kim J, Nam B, Kim GY, Lee S, Seo S and Jo W: Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity. Int J Mol Med 31: 205-212, 2013
APA
Jeong, M., Yang, K., Kim, J., Nam, B., Kim, G., Lee, S. ... Jo, W. (2013). Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity. International Journal of Molecular Medicine, 31, 205-212. https://doi.org/10.3892/ijmm.2012.1181
MLA
Jeong, M., Yang, K., Kim, J., Nam, B., Kim, G., Lee, S., Seo, S., Jo, W."Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity". International Journal of Molecular Medicine 31.1 (2013): 205-212.
Chicago
Jeong, M., Yang, K., Kim, J., Nam, B., Kim, G., Lee, S., Seo, S., Jo, W."Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity". International Journal of Molecular Medicine 31, no. 1 (2013): 205-212. https://doi.org/10.3892/ijmm.2012.1181