Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells

  • Authors:
    • Yun-Hee Sung
    • Mal-Soon Shin
    • Il-Gyu Ko
    • Sung-Eun Kim
    • Chang-Ju Kim
    • Hyun-Jong Ahn
    • Hye-Sun Yoon
    • Bong-Jae Lee
  • View Affiliations

  • Published online on: March 27, 2013     https://doi.org/10.3892/ijmm.2013.1322
  • Pages: 1030-1036
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Abstract

Ulinastatin is an intrinsic serine-protease urinary trypsin inhibitor that can be extracted and purified from human urine. Urinary trypsin inhibitors are widely used to treat patients with acute inflammatory disorders, such as shock and pancreatitis. However, although the anti-inflammatory activities of urinary trypsin inhibitors have been investigated, the mechanisms underlying their actions are not yet fully understood. In the present study, we evaluated the effect of ulinastatin on lipopolysaccharide (LPS)-induced inflammation in relation with nuclear factor-κB (NF-κB) activation using BV2 mouse microglial cells. To accomplish this, we performed a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis, electrophoretic mobility gel shift assay (EMSA), prostaglandin E2 (PGE2) immunoassay and nitric oxide (NO) detection. The results demonstrated that ulinastatin suppressed PGE2 synthesis and NO production by inhibiting the LPS-induced mRNA and protein expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) in BV2 mouse microglial cells. Ulinastatin suppressed the activation of NF-κB in the nucleus. These findings demonstrate that ulinastatin exerts analgesic and anti-inflammatory effects that possibly occur via the suppression of COX-2 and iNOS expression through the downregulation of NF-κB activity.
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May 2013
Volume 31 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sung Y, Shin M, Ko I, Kim S, Kim C, Ahn H, Yoon H and Lee B: Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. Int J Mol Med 31: 1030-1036, 2013
APA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H. ... Lee, B. (2013). Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. International Journal of Molecular Medicine, 31, 1030-1036. https://doi.org/10.3892/ijmm.2013.1322
MLA
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31.5 (2013): 1030-1036.
Chicago
Sung, Y., Shin, M., Ko, I., Kim, S., Kim, C., Ahn, H., Yoon, H., Lee, B."Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells". International Journal of Molecular Medicine 31, no. 5 (2013): 1030-1036. https://doi.org/10.3892/ijmm.2013.1322