Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments

  • Authors:
    • Chuan Cai
    • Gong-Jie Yuan
    • Ye Huang
    • Nan Yang
    • Xin Chen
    • Li Wen
    • Xi Wang
    • Liang Zhang
    • Yin Ding
  • View Affiliations

  • Published online on: March 15, 2013     https://doi.org/10.3892/ijmm.2013.1305
  • Pages: 1195-1201
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Abstract

Recently, it has been reported that the orphan nuclear receptor estrogen-related receptor α (ERRα) is involved in the osteogenic differentiation of mesenchymal stem cells (MSCs). Moreover, ERRα has been identified as a novel therapeutic target for treating osteoporosis and other bone diseases. Human periodontal ligament tissue-derived mesenchymal stem cells (hPDLSCs) have recently been used in stem cell-mediated therapies because of their multipotency, particularly toward osteogenic differentiation. However, it is still unclear whether ERRα can regulate the osteogenic differentiation of hPDLSCs. In the present study, we investigated the role of ERRα in the osteogenic differentiation of hPDLSCs in vitro. We isolated hPDLSCs and confirmed their capacity for multipotent differentiation. Furthermore, we examined ERRα expression in hPDLSCs by RT-PCR and immunocytochemistry. We found that the expression of ERRα mRNA was significantly increased during the late stage of osteogenic differentiation of hPDLSCs. Moreover, transfection of recombinant lentiviral-mediated miRNA targeting ERRα significantly suppressed ALP activity, mineralization capacity, and the mRNA expression of osteogenesis-related genes (ALP, OCN, RUNX2 and OPN) in hPDLSCs. Our results indicate that ERRα may promote the osteogenic differentiation of hPDLSCs in vitro.
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May 2013
Volume 31 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cai C, Yuan G, Huang Y, Yang N, Chen X, Wen L, Wang X, Zhang L and Ding Y: Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. Int J Mol Med 31: 1195-1201, 2013
APA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L. ... Ding, Y. (2013). Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. International Journal of Molecular Medicine, 31, 1195-1201. https://doi.org/10.3892/ijmm.2013.1305
MLA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31.5 (2013): 1195-1201.
Chicago
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31, no. 5 (2013): 1195-1201. https://doi.org/10.3892/ijmm.2013.1305