Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene

  • Authors:
    • Juanjuan Qiu
    • Daiqing Wang
    • Yufang Ma
    • Tao Jiang
    • Yi Xin
  • View Affiliations

  • Published online on: March 12, 2013     https://doi.org/10.3892/ijmm.2013.1298
  • Pages: 1229-1233
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Serine acetyltransferase (CysE) is the first enzyme involved in the two-step enzymatic pathway of L-cysteine biosynthesis in bacteria and plants, but not in humans. CysE catalyzes the biosynthesis of O-acetyl-L-serine and CoA from L-serine (L-Ser) and acetyl-CoA (AcCoA). Mycobacterium tuberculosis (M. tuberculosis) Rv2335 was predicted as the cysE gene encoding serine acetyltransferase. In this study, the M. tuberculosis Rv2335 gene was cloned and the CysE protein was expressed in E. coli BL21 (DE3). The M. tuberculosis CysE protein was purified by Ni2+ affinity chromatography and confirmed by SDS-PAGE, western blotting and mass spectrometry. The serine acetyltransferase activity of the M. tuberculosis CysE protein was detected using Ellman's reagent. M. tuberculosis CysE displayed optimal activity at pH 7.5 and 37˚C. The Michaelis constant for AcCoA and L-Ser was 0.0513±0.0050 and 0.0264±0.0006 mM, respectively. The maximum velocity (Vmax) for CysE was 0.0073±0.0005 mM/min. The CysE assay and the determination of the kinetic parameters of M. tuberculosis CysE may be helpful for screening its inhibitors in anti-tuberculosis drug discovery.
View Figures
View References

Related Articles

Journal Cover

May 2013
Volume 31 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Qiu J, Wang D, Ma Y, Jiang T and Xin Y: Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene. Int J Mol Med 31: 1229-1233, 2013
APA
Qiu, J., Wang, D., Ma, Y., Jiang, T., & Xin, Y. (2013). Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene. International Journal of Molecular Medicine, 31, 1229-1233. https://doi.org/10.3892/ijmm.2013.1298
MLA
Qiu, J., Wang, D., Ma, Y., Jiang, T., Xin, Y."Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene". International Journal of Molecular Medicine 31.5 (2013): 1229-1233.
Chicago
Qiu, J., Wang, D., Ma, Y., Jiang, T., Xin, Y."Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene". International Journal of Molecular Medicine 31, no. 5 (2013): 1229-1233. https://doi.org/10.3892/ijmm.2013.1298