Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets: Importance of pre-treatment

  • Authors:
    • Nguyen The Cuong
    • Tomoaki Doi
    • Rie Matsushima-Nishiwaki
    • Shigeru Akamatsu
    • Gen Kuroyanagi
    • Akira Kondo
    • Jun Mizutani
    • Ikuo Wada
    • Takanobu Otsuka
    • Haruhiko Tokuda
    • Osamu Kozawa
    • Shinji Ogura
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  • Published online on: April 11, 2013     https://doi.org/10.3892/ijmm.2013.1345
  • Pages: 1291-1297
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Abstract

It has been shown that thrombopoietin (TPO) amplifies agonist-induced platelet activation. However, the precise mechanism of action of TPO has not yet been fully elucidated. We have previously reported that the adenosine diphosphate (ADP)‑induced phosphorylation of heat shock protein 27 (HSP27) via the p38 mitogen-activated protein (MAP) kinase pathway correlates with the ADP-induced platelet-derived growth factor (PDGF)-AB secretion and the release of soluble CD40 ligand (sCD40L) from human platelets. In the present study, we investigated the effects of TPO on platelet activation induced by ADP. We examined the effects of TPO on ADP-induced platelet activation under different treatments: TPO was administered 15 min prior to stimulation with ADP (pre-treatment); TPO and ADP were simultaneously administered (simultaneous treatment); and TPO was administered 2 min following stimulation with ADP (post-treatment). TPO, which alone had no effect on platelet aggregation, synergistically enhanced the ADP (1 mM)-induced platelet aggregation only when it was administered prior to stimulation with ADP. Pre-treatment with TPO significantly increased the secretion of PDGF-AB and the release of sCD40L, and markedly enhanced the ADP-induced phosphorylation of p38 MAP kinase and HSP27 in the platelets. However, simultaneous treatment with TPO or TPO post-treatment failed to affect the ADP-induced platelet aggregation, the secretion of PDGF-AB, the release of sCD40L and the phosphorylation p38 MAP kinase or HSP27. These results strongly suggest that pre-treatment with TPO significantly amplifies ADP-induced HSP27 phosphorylation via the p38 MAP kinase pathway in human platelets.
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June 2013
Volume 31 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cuong NT, Doi T, Matsushima-Nishiwaki R, Akamatsu S, Kuroyanagi G, Kondo A, Mizutani J, Wada I, Otsuka T, Tokuda H, Tokuda H, et al: Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets: Importance of pre-treatment. Int J Mol Med 31: 1291-1297, 2013
APA
Cuong, N.T., Doi, T., Matsushima-Nishiwaki, R., Akamatsu, S., Kuroyanagi, G., Kondo, A. ... Ogura, S. (2013). Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets: Importance of pre-treatment. International Journal of Molecular Medicine, 31, 1291-1297. https://doi.org/10.3892/ijmm.2013.1345
MLA
Cuong, N. T., Doi, T., Matsushima-Nishiwaki, R., Akamatsu, S., Kuroyanagi, G., Kondo, A., Mizutani, J., Wada, I., Otsuka, T., Tokuda, H., Kozawa, O., Ogura, S."Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets: Importance of pre-treatment". International Journal of Molecular Medicine 31.6 (2013): 1291-1297.
Chicago
Cuong, N. T., Doi, T., Matsushima-Nishiwaki, R., Akamatsu, S., Kuroyanagi, G., Kondo, A., Mizutani, J., Wada, I., Otsuka, T., Tokuda, H., Kozawa, O., Ogura, S."Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets: Importance of pre-treatment". International Journal of Molecular Medicine 31, no. 6 (2013): 1291-1297. https://doi.org/10.3892/ijmm.2013.1345