miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression

  • Authors:
    • Min Kang
    • Yumei Li
    • Wenqi Liu
    • Rensheng Wang
    • Anzhou Tang
    • Hong Hao
    • Zhenguo Liu
    • Hesheng Ou
  • View Affiliations

  • Published online on: May 16, 2013     https://doi.org/10.3892/ijmm.2013.1384
  • Pages: 51-58
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Abstract

We report the emerging role of microRNA (miRNA) deregulation associated with activation of an oncogene SOX4 (a member of the SRY-related HMG-box) in esophageal carcinoma. Paired esophageal cancer and adjacent non-tumor tissues were obtained from 42 patients who underwent primary surgical resection for esophageal cancer. Experiments such as real-time PCR, western blot analysis, luciferase-reporter assay, cell proliferation and colony formation assays, in vitro migration and invasion assays, and a wound-healing assay were performed to determine the effects of miR-129-2. We found that SOX4 expression was elevated (P<0.005) in esophageal tumors (n=42) when compared with its expression in the controls (n=42). Compared with the normal esophageal tissues, the expression of miR-129-2 was downregulated in 27 of 31 primary esophageal tumors, while the expression of SOX4 was upregulated (P<0.001). Restoration of miR-129-2 by transfection with an miRNA expression plasmid led to a decrease in SOX4 expression, which was accompanied by reduced migration and proliferation of the cancer cells. These results suggest that aberrant expression of SOX4 is associated with repression of miR-129-2, and restoration of miR-129-2 suppresses the migration and proliferation of esophageal cancer cells. Our results demonstrated that the deregulation of miR-129-2 leads to aberrant SOX4 expression, presenting a new paradigm in which the restoration of miRNA suppresses its oncogenic target in esophageal cancer.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kang M, Li Y, Liu W, Wang R, Tang A, Hao H, Liu Z and Ou H: miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. Int J Mol Med 32: 51-58, 2013
APA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H. ... Ou, H. (2013). miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. International Journal of Molecular Medicine, 32, 51-58. https://doi.org/10.3892/ijmm.2013.1384
MLA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32.1 (2013): 51-58.
Chicago
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32, no. 1 (2013): 51-58. https://doi.org/10.3892/ijmm.2013.1384