Sex determining region Y-box 2 inhibits the proliferation of colorectal adenocarcinoma cells through the mTOR signaling pathway

  • Authors:
    • Hui Liu
    • Lutao Du
    • Zhihua Wen
    • Yongmei Yang
    • Juan Li
    • Zhaogang Dong
    • Guixi Zheng
    • Lili Wang
    • Xin Zhang
    • Chuanxin Wang
  • View Affiliations

  • Published online on: April 19, 2013     https://doi.org/10.3892/ijmm.2013.1354
  • Pages: 59-66
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Abstract

Sex determining region Y-box 2 (SOX2), is a high mobility group box transcription factor involved in the maintenance of pluripotency and the self-renewal of embryonic and neuronal stem cells, which also plays differential roles in the cell proliferation of several tumors. However, its role in colorectal adenocarcinoma cell proliferation and the underlying mechanisms remain unclear. The mammalian target of rapamycin (mTOR) signaling pathway has recently emerged as an important regulator of cell proliferation in many types of cancer. In this study, we examined the effect of SOX2 on the proliferation of colorectal adenocarcinoma cells and evaluated the role of the mTOR pathway in this process. Our results indicated that the overexpression of SOX2 significantly inhibited the proliferation of colorectal adenocarcinoma cells. Of note, mechanistic investigations revealed that SOX2 inhibited the activation of the mTOR pathway in HT-29 cells. We then examined the effect of SOX2 on the cell cycle, and the results revealed that SOX2 downregulated cyclin D1 expression and induced G0/G1 arrest in the HT-29 cells. Moreover, we also analyzed the correlation between SOX2 expression and patient clinicopathological characteristics in colorectal adenocarcinoma tissues, as well as the level of phospho-S6 (S235/236), phospho-Akt (S473) and cyclin D1. The results revealed a significant negative correlation between SOX2 expression and tumor size and the levels of phospho-S6 (S235/236), phospho-Akt (S473) and cyclin D1. Taken together, to our knowledge, our findings suggest for the first time that SOX2 suppresses colorectal adenocarcinoma cell proliferation through the inhibition of the mTOR pathway.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liu H, Du L, Wen Z, Yang Y, Li J, Dong Z, Zheng G, Wang L, Zhang X, Wang C, Wang C, et al: Sex determining region Y-box 2 inhibits the proliferation of colorectal adenocarcinoma cells through the mTOR signaling pathway. Int J Mol Med 32: 59-66, 2013
APA
Liu, H., Du, L., Wen, Z., Yang, Y., Li, J., Dong, Z. ... Wang, C. (2013). Sex determining region Y-box 2 inhibits the proliferation of colorectal adenocarcinoma cells through the mTOR signaling pathway. International Journal of Molecular Medicine, 32, 59-66. https://doi.org/10.3892/ijmm.2013.1354
MLA
Liu, H., Du, L., Wen, Z., Yang, Y., Li, J., Dong, Z., Zheng, G., Wang, L., Zhang, X., Wang, C."Sex determining region Y-box 2 inhibits the proliferation of colorectal adenocarcinoma cells through the mTOR signaling pathway". International Journal of Molecular Medicine 32.1 (2013): 59-66.
Chicago
Liu, H., Du, L., Wen, Z., Yang, Y., Li, J., Dong, Z., Zheng, G., Wang, L., Zhang, X., Wang, C."Sex determining region Y-box 2 inhibits the proliferation of colorectal adenocarcinoma cells through the mTOR signaling pathway". International Journal of Molecular Medicine 32, no. 1 (2013): 59-66. https://doi.org/10.3892/ijmm.2013.1354