COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer

Corrigendum in: /10.3892/ijmm.2022.5083

  • Authors:
    • Zhaofeng Chen
    • Min Liu
    • Xiaojun Liu
    • Shanshan Huang
    • Linlin Li
    • Bo Song
    • Hailong Li
    • Qian Ren
    • Zenan Hu
    • Yongning Zhou
    • Liang Qiao
  • View Affiliations

  • Published online on: May 10, 2013     https://doi.org/10.3892/ijmm.2013.1376
  • Pages: 93-100
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Abstract

Cyclooxygenase-2 (COX-2) participates in cancer invasion and metastasis by decreasing the expression of E-cadherin. However, the molecular mechanisms through which COX-2 regulates E-cadherin expression and function have not yet been fully elucidated. The aim of this study was to investigate the possible molecular mechanisms through which COX-2 regulates E-cadherin expression in gastric cancer. The mRNA and protein expression of COX-2, nuclear factor-κB (NF-κB), Snail and E-cadherin was detected in gastric cancer cells by quantitative PCR and western blot analysis, respectively. The expression of these genes was also detected in healthy gastric mucosa and gastric cancer tissues by immunohistochemistry. We detected various levels of COX-2, nuclear factor-κB (NF-κB), Snail and E-cadherin expression in the normal gastric mucosa and cancer tissues; however, the expression patterns differed: the increased expression of COX-2, NF-κB and Snail was observed in the gastric cancer tissues, whereas there was a considerable reduction in E-cadherin expression in the cancer tissues compared to the normal gastric mucosa. The expression patterns of COX-2, NF-κB and Snail were similar. The increased expression of COX-2 in the gastric cancer tissues closely correlated with the increased expression of NF-κB and Snail, but inversely correlated with the expression of E-cadherin. Treatment of the SGC7901 cells (which express high levels of COX-2) with celecoxib, a COX-2 inhibitor, not only led to a marked dose- and time-dependent decrease in the expression of COX-2, NF-κB and Snail, but also led to a significant increase in the expression of E-cadherin, and this was associated with a reduction in cell invasion. By contrast, the same treatment did not alter the expression of these genes in another gastric cancer cell line, MGC803 (which barely expresses COX-2). These data suggest that COX-2 regulates the expression of E-cadherin through the NF-κB and Snail signaling pathway in gastric cancer.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Chen Z, Liu M, Liu X, Huang S, Li L, Song B, Li H, Ren Q, Hu Z, Zhou Y, Zhou Y, et al: COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer Corrigendum in /10.3892/ijmm.2022.5083. Int J Mol Med 32: 93-100, 2013
APA
Chen, Z., Liu, M., Liu, X., Huang, S., Li, L., Song, B. ... Qiao, L. (2013). COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer Corrigendum in /10.3892/ijmm.2022.5083. International Journal of Molecular Medicine, 32, 93-100. https://doi.org/10.3892/ijmm.2013.1376
MLA
Chen, Z., Liu, M., Liu, X., Huang, S., Li, L., Song, B., Li, H., Ren, Q., Hu, Z., Zhou, Y., Qiao, L."COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer Corrigendum in /10.3892/ijmm.2022.5083". International Journal of Molecular Medicine 32.1 (2013): 93-100.
Chicago
Chen, Z., Liu, M., Liu, X., Huang, S., Li, L., Song, B., Li, H., Ren, Q., Hu, Z., Zhou, Y., Qiao, L."COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer Corrigendum in /10.3892/ijmm.2022.5083". International Journal of Molecular Medicine 32, no. 1 (2013): 93-100. https://doi.org/10.3892/ijmm.2013.1376