Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis

  • Authors:
    • Guohong Cao
    • Jinjin Zhang
    • Meirong Wang
    • Xiaodong Song
    • Wenbo Liu
    • Cuiping Mao
    • Changjun Lv
  • View Affiliations

  • Published online on: June 4, 2013     https://doi.org/10.3892/ijmm.2013.1404
  • Pages: 355-364
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Abstract

Recent studies suggest that long non‑coding RNAs (lncRNAs) are more involved in human diseases than previously realized. A growing body of evidence links lncRNA mutation and dysregulation to diverse human diseases. However, the association of lncRNAs with the pathogenesis of lung fibrosis remains poorly understood. In this study, we detected changes in hydroxyproline and collagen levels, as well as the ultrastructure of lung tissue to develop a rat model of lung fibrosis. The differentially expressed lncRNAs and mRNA profiles between fibrotic lung and normal lung tissue were analyzed using microarrays. Gene Ontology analysis and pathway analysis were performed for further research. Two differentially expressed lncRNAs, namely, AJ005396 and S69206, were detected by in situ hybridization to validate the microarray data. The results revealed that the number of collagen fibers in the interstitial lung tissue significantly increased in the model group compared with the normal group. In total, 210 and 358 lncRNAs were upregulated and downregulated, respectively, along with 415 upregulated and 530 downregulated mRNAs in the rats with lung fibrosis. AJ005396 and S69206 were upregulated in the fibrotic lung tissue, consistent with the microarray data, and were located in the cytoplasm of the interstitial lung cells. In conclusion, the expression profile of the lncRNAs was significantly altered in the fibrotic lung tissue and these transcripts are potential molecular targets for inhibiting the development of lung fibrosis.
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August 2013
Volume 32 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cao G, Zhang J, Wang M, Song X, Liu W, Mao C and Lv C: Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis. Int J Mol Med 32: 355-364, 2013
APA
Cao, G., Zhang, J., Wang, M., Song, X., Liu, W., Mao, C., & Lv, C. (2013). Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis. International Journal of Molecular Medicine, 32, 355-364. https://doi.org/10.3892/ijmm.2013.1404
MLA
Cao, G., Zhang, J., Wang, M., Song, X., Liu, W., Mao, C., Lv, C."Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis". International Journal of Molecular Medicine 32.2 (2013): 355-364.
Chicago
Cao, G., Zhang, J., Wang, M., Song, X., Liu, W., Mao, C., Lv, C."Differential expression of long non-coding RNAs in bleomycin-induced lung fibrosis". International Journal of Molecular Medicine 32, no. 2 (2013): 355-364. https://doi.org/10.3892/ijmm.2013.1404