miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1

  • Authors:
    • Haosha Tang
    • Liangqing Yao
    • Xiang Tao
    • Yi Yu
    • Mo Chen
    • Rong Zhang
    • Congjian Xu
  • View Affiliations

  • Published online on: May 30, 2013     https://doi.org/10.3892/ijmm.2013.1400
  • Pages: 381-388
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Abstract

microRNAs (miRNAs) are important regulators of gene expression during tumorigenesis. The downregulation of microRNA-9 (miR-9) has been reported in ovarian serous carcinoma (OSC), indicating a role for miR-9 in this type of cancer. In this study, we investigated the biological significance of miR-9 in OSC in vitro. Using 3 OSC cell lines, SKOV3, CAOV3 and OVCAR3, which underexpresss miR-9, we demonstrate that the exogenous miR-9 transfection inhibits OSC cell proliferation, migration and invasion. In addition, we demonstrate that the focal adhesion protein, talin 1 (TLN1), whose expression has been associated with OSC development and progression to metastasis, is a direct target of miR-9. TLN1 knockdown mimicked the effects of miR-9 overexpression. Moreover, the activation of the TLN1-modulated FAK/AKT pathway was inhibited by the increased miR-9 levels. These results suggest that miR-9 plays a role as a tumor suppressor in OSC by suppressing TLN1 expression.
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August 2013
Volume 32 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tang H, Yao L, Tao X, Yu Y, Chen M, Zhang R and Xu C: miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1. Int J Mol Med 32: 381-388, 2013
APA
Tang, H., Yao, L., Tao, X., Yu, Y., Chen, M., Zhang, R., & Xu, C. (2013). miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1. International Journal of Molecular Medicine, 32, 381-388. https://doi.org/10.3892/ijmm.2013.1400
MLA
Tang, H., Yao, L., Tao, X., Yu, Y., Chen, M., Zhang, R., Xu, C."miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1". International Journal of Molecular Medicine 32.2 (2013): 381-388.
Chicago
Tang, H., Yao, L., Tao, X., Yu, Y., Chen, M., Zhang, R., Xu, C."miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1". International Journal of Molecular Medicine 32, no. 2 (2013): 381-388. https://doi.org/10.3892/ijmm.2013.1400