Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo

  • Authors:
    • Jian Zhou
    • Shiduo Song
    • Songbin He
    • Zhenxin Wang
    • Bing Zhang
    • Dechun Li
    • Dongming Zhu
  • View Affiliations

  • Published online on: July 11, 2013     https://doi.org/10.3892/ijmm.2013.1437
  • Pages: 653-660
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Decoy receptor 3 (DcR3) is abundantly expressed in human tumors and protects cells from a wide range of apoptotic stimuli. In this study, we demonstrate that DcR3 is overexpressed in pancreatic carcinoma cells, and that the pancreatic carcinoma cell lines, Panc-1 and SW1990, are resistant to Fas ligand (FasL)-mediated apoptosis. To further define the function of DcR3 in cell growth and apoptosis, we used small interfering RNA (siRNA) to knockdown the expression of the DcR3 gene in Panc-1 and SW1990 cells. Our results revealed that the silencing of DcR3 expression enhanced the inhibitory effects of FasL and reduced the capabiltiy of the cells for proliferation and colony formation in vitro. In addition, the downregulation of DcR3 modulated the cell apoptotic regulators, Fas-associated death domain (FADD), caspase‑3 and caspase‑8, thus triggering cell apoptosis. Furthermore, the knockdown of DcR3 inhibited the growth of Panc-1 tumor xenografts. Taken together, our findings indicate that DcR3 is important in cancer progression and may be a used as a potential therapeutic target for the gene therapy of pancreatic carcinoma.
View Figures
View References

Related Articles

Journal Cover

September 2013
Volume 32 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Song S, He S, Wang Z, Zhang B, Li D and Zhu D: Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo. Int J Mol Med 32: 653-660, 2013
APA
Zhou, J., Song, S., He, S., Wang, Z., Zhang, B., Li, D., & Zhu, D. (2013). Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo. International Journal of Molecular Medicine, 32, 653-660. https://doi.org/10.3892/ijmm.2013.1437
MLA
Zhou, J., Song, S., He, S., Wang, Z., Zhang, B., Li, D., Zhu, D."Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo". International Journal of Molecular Medicine 32.3 (2013): 653-660.
Chicago
Zhou, J., Song, S., He, S., Wang, Z., Zhang, B., Li, D., Zhu, D."Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo". International Journal of Molecular Medicine 32, no. 3 (2013): 653-660. https://doi.org/10.3892/ijmm.2013.1437