Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes

  • Authors:
    • Un Suk Jung
    • Kang Jin Jeong
    • Jae Ku Kang
    • Kyongwook Yi
    • Jung-Ho Shin
    • Hong Seog Seo
    • Tak Kim
    • Sun-Haeng Kim
    • Jun-Young Hur
  • View Affiliations

  • Published online on: July 12, 2013     https://doi.org/10.3892/ijmm.2013.1440
  • Pages: 723-728
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Abstract

The aim of this study was to investigate the effects of estrogen and estrogen receptor α (ERα) and β (ERβ) on the expression of visfatin and retinol-binding protein 4 (RBP4) by treating 3T3-L1 adipocytes with estradiol (E2), estrogen receptor agonists and antagonists. Mature adipocytes were exposed to E2, the ERα agonist, 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), the ERβ agonist, 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), E2 with the ERα antagonist, 1,3-bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride (MPP), and E2 with the ERβ antagonist, (5R, 11R)-5,11-diethyl-5,6,11,12-tetrahydro-2,8-chrysenediol [(R,R)-THC], at various concentrations. To determine the effects of ER subtypes on the expression of adipokines, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analyses were performed. E2 concentrations of 10-5 and 10-6 mol/l induced a statistically significant increase in the expression of RBP4 (P=0.012 and P=0.011, respectively). In the cells treated with 10-5 mol/l PPT, RBP4 expression significantly increased (P<0.05) in a dose-dependent manner. Treatment with the ERα antagonist, MPP (10-5 mol/l), and E2 suppressed the expression of RBP4 (P=0.032). However, the expression of RBP4 was not significantly altered when the cells were treated with the ERβ agonist or antagonist. The expression of visfatin was not affected by different concentrations of E2 and ERs. 17β-estradiol significantly increased the secretion of RBP4 and upregulated RBP4 expression via ERα but not ERβ in 3T3-L1 adipocytes. RBP4 expression was regulated by estrogen in the 3T3-L1 adipocytes and this effect was selectively mediated by ERα.
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September 2013
Volume 32 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jung US, Jeong KJ, Kang JK, Yi K, Shin J, Seo HS, Kim T, Kim S and Hur J: Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes. Int J Mol Med 32: 723-728, 2013
APA
Jung, U.S., Jeong, K.J., Kang, J.K., Yi, K., Shin, J., Seo, H.S. ... Hur, J. (2013). Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes. International Journal of Molecular Medicine, 32, 723-728. https://doi.org/10.3892/ijmm.2013.1440
MLA
Jung, U. S., Jeong, K. J., Kang, J. K., Yi, K., Shin, J., Seo, H. S., Kim, T., Kim, S., Hur, J."Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes". International Journal of Molecular Medicine 32.3 (2013): 723-728.
Chicago
Jung, U. S., Jeong, K. J., Kang, J. K., Yi, K., Shin, J., Seo, H. S., Kim, T., Kim, S., Hur, J."Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes". International Journal of Molecular Medicine 32, no. 3 (2013): 723-728. https://doi.org/10.3892/ijmm.2013.1440