Silencing of angiotensin‑converting enzyme by RNA interference prevents H9c2 cardiomyocytes from apoptosis induced by anoxia/reoxygenation through regulation of the intracellular renin-angiotensin system

  • Authors:
    • Weiguo Wan
    • Xuejun Jiang
    • Xiaoyan Li
    • Cui Zhang
    • Xin Yi
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  • Published online on: October 11, 2013     https://doi.org/10.3892/ijmm.2013.1525
  • Pages: 1380-1386
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Abstract

Inhibition of the angiotensin‑converting enzyme (ACE) attenuated apoptotic cardiomyocytes induced by ischemic reperfusion (I/R). However, it is difficult to evaluate the effects of inhibition of the intracellular ACE in vivo. The objective of this study was to determine whether the apoptosis in H9c2 cardiomyocytes following anoxia/reoxygenation (A/R) would be improved by the silencing of intracellular ACE by RNA interference (RNAi). H9c2 cardiomyocytes were subjected to A/R 48 h following transfection with ACE-shRNA plasmid. The results showed that the gene silencing of intracellular ACE significantly inhibited the decrease of cell viability and the increase of apoptotic H9c2 cardiomyocytes undergoing A/R. Additionally, the gene silencing of intracellular ACE significantly promoted the expression of ACE2, decreased caspase‑3 activity and Bax levels, and enhanced the expression of Bcl‑2 in H9c2 cardiomyocytes subjected to A/R. The results suggest that the gene silencing of intracellular ACE holds great potential in the treatment of cardiomyocyte apoptosis following I/R injury through the regulation of the intracellular renin‑angiotensin system, thereby regulating the intrinsic pathway of apoptosis.
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December 2013
Volume 32 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wan W, Jiang X, Li X, Zhang C and Yi X: Silencing of angiotensin‑converting enzyme by RNA interference prevents H9c2 cardiomyocytes from apoptosis induced by anoxia/reoxygenation through regulation of the intracellular renin-angiotensin system. Int J Mol Med 32: 1380-1386, 2013
APA
Wan, W., Jiang, X., Li, X., Zhang, C., & Yi, X. (2013). Silencing of angiotensin‑converting enzyme by RNA interference prevents H9c2 cardiomyocytes from apoptosis induced by anoxia/reoxygenation through regulation of the intracellular renin-angiotensin system. International Journal of Molecular Medicine, 32, 1380-1386. https://doi.org/10.3892/ijmm.2013.1525
MLA
Wan, W., Jiang, X., Li, X., Zhang, C., Yi, X."Silencing of angiotensin‑converting enzyme by RNA interference prevents H9c2 cardiomyocytes from apoptosis induced by anoxia/reoxygenation through regulation of the intracellular renin-angiotensin system". International Journal of Molecular Medicine 32.6 (2013): 1380-1386.
Chicago
Wan, W., Jiang, X., Li, X., Zhang, C., Yi, X."Silencing of angiotensin‑converting enzyme by RNA interference prevents H9c2 cardiomyocytes from apoptosis induced by anoxia/reoxygenation through regulation of the intracellular renin-angiotensin system". International Journal of Molecular Medicine 32, no. 6 (2013): 1380-1386. https://doi.org/10.3892/ijmm.2013.1525