Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy

  • Authors:
    • Jiu-Cong Zhang
    • Fang Xie
    • Xiao-Hui Yu
    • Zhi-Yun Deng
    • Yong Wang
    • Ping Liang
    • Li Sun
    • Fang-Xin Zhang
  • View Affiliations

  • Published online on: December 11, 2013     https://doi.org/10.3892/ijmm.2013.1584
  • Pages: 431-440
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Abstract

In this study, we aimed to measure P-glycoprotein (P-gp) expression in CD8+ T lymphocytes of HIV-1-infected patients, to investigate how P-gp levels are affected by antiretroviral therapy (ART) in HIV-1 infection, and to assess the value of using P-gp expression to predict virologic response to ART. Peripheral blood mononuclear cells (PBMCs) were obtained from a cohort of HIV-1‑infected patients in China: 140 patients on ART, and 49 ART-naïve patients. We also enrolled 24 healthy blood donors as the controls. The expression levels of P-gp in CD8+ T cells of HIV-1-infected patients were evaluated by quantitative reverse transcription PCR, ELISA and flow cytometry. A high inter-individual variability was observed in the CD8+ T cells of both HIV-1-infected patients and healthy donors; however, the expression levels of P-gp were significantly higher in the HIV-1-infected group on ART compared to the ART-naïve group. The relative proportion of P-gp+CD8+ T cells inversely correlated with the blood CD4+ T cell count in the HIV-1‑infected patients on ART (r=-0.3343, P=0.0375). Groups of both good and poor responders showed significantly elevated levels of P-gp+CD8+ T cells. The percentage of P-gp+CD8+ T cells appeared to provide a sensitive estimate of the virologic response to ART compared to the CD4+ T cell count. Our results suggest that P-gp expression varies among HIV-1‑infected patients, but is significantly higher in HIV-1‑infected patients on ART. The overexpression of P-gp is involved in ART initiation during HIV-1 infection, and P-gp+CD8+ T cells may be an additional criterion for the evaluation of the antiretroviral response to ART.
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2014-February
Volume 33 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang J, Xie F, Yu X, Deng Z, Wang Y, Liang P, Sun L and Zhang F: Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy. Int J Mol Med 33: 431-440, 2014
APA
Zhang, J., Xie, F., Yu, X., Deng, Z., Wang, Y., Liang, P. ... Zhang, F. (2014). Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy. International Journal of Molecular Medicine, 33, 431-440. https://doi.org/10.3892/ijmm.2013.1584
MLA
Zhang, J., Xie, F., Yu, X., Deng, Z., Wang, Y., Liang, P., Sun, L., Zhang, F."Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy". International Journal of Molecular Medicine 33.2 (2014): 431-440.
Chicago
Zhang, J., Xie, F., Yu, X., Deng, Z., Wang, Y., Liang, P., Sun, L., Zhang, F."Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy". International Journal of Molecular Medicine 33, no. 2 (2014): 431-440. https://doi.org/10.3892/ijmm.2013.1584