Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells

  • Authors:
    • Emiko Aomatsu
    • Naoyuki Chosa
    • Soko Nishihira
    • Yoshiki Sugiyama
    • Hiroyuki Miura
    • Akira Ishisaki
  • View Affiliations

  • Published online on: December 27, 2013     https://doi.org/10.3892/ijmm.2013.1607
  • Pages: 565-572
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Abstract

Cell-cell adhesions induce various intracellular signals through hierarchical and synergistic molecular interactions. Recently, we demonstrated that a high cell density induces the expression of vascular cell adhesion molecule-1 (VCAM-1) through the nuclear factor-κB (NF-κB) pathway in human bone marrow-derived mesenchymal stem cells (MSCs). However, the specific molecules that activated the NF-κB pathway were not determined. In the present study, in experiments with receptor tyrosine kinase inhibitors, VCAM-1 expression was completely suppressed by platelet-derived growth factor (PDGF) receptor (PDGFR) inhibitors. In addition, VCAM-1 expression was significantly suppressed by knockdown with PDGFRβ siRNA, but not with PDGFRα siRNA. However, VCAM-1 expression did not increase following treatment with PDGF. The overexpression of N-cadherin, a structural molecule in adherence junctions in MSCs, promoted VCAM-1 expression and induced the marked phosphorylation of the intracellular signaling factor, Src. In addition, VCAM-1 expression and Src phosphorylation were reduced by the overexpression of a dominant negative mutant of N-cadherin. These results suggest that cell-cell adhesion, through N-cadherin, enhances the expression of VCAM-1 via PDGFRβ and the activation of Src in a ligand-independent manner in MSCs.
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2014-March
Volume 33 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Aomatsu E, Chosa N, Nishihira S, Sugiyama Y, Miura H and Ishisaki A: Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells. Int J Mol Med 33: 565-572, 2014
APA
Aomatsu, E., Chosa, N., Nishihira, S., Sugiyama, Y., Miura, H., & Ishisaki, A. (2014). Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells. International Journal of Molecular Medicine, 33, 565-572. https://doi.org/10.3892/ijmm.2013.1607
MLA
Aomatsu, E., Chosa, N., Nishihira, S., Sugiyama, Y., Miura, H., Ishisaki, A."Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells". International Journal of Molecular Medicine 33.3 (2014): 565-572.
Chicago
Aomatsu, E., Chosa, N., Nishihira, S., Sugiyama, Y., Miura, H., Ishisaki, A."Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells". International Journal of Molecular Medicine 33, no. 3 (2014): 565-572. https://doi.org/10.3892/ijmm.2013.1607