Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

  • Authors:
    • Hwa Jun Cha
    • Kwang Sik Lee
    • Ghang Tai Lee
    • Kun Kook Lee
    • Jin Tae Hong
    • Sung Nae Lee
    • Hyun Hee Jang
    • Jae Ho Lee
    • In-Chul Park
    • Yu Ri Kim
    • Kyu Joong Ahn
    • Seung Bin Kwon
    • In-Sook An
    • Sungkwan An
    • Seunghee Bae
  • View Affiliations

  • Published online on: February 7, 2014     https://doi.org/10.3892/ijmm.2014.1647
  • Pages: 957-963
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Abstract

The aim of this study was to investigate the mechanisms by which troxerutin protects cells against ultraviolet B (UVB) radiation. First, we demonstrate that pre-treatment with troxerutin protects normal human dermal fibroblasts (nHDFs) against UVB-induced cytotoxicity. As shown by migration assay and DNA repair analysis, troxerutin increased cell migration and DNA repair activity in the nHDFs. Subsequently, we analyzed microRNA (miRNA) expression profiles in the nHDFs. miRNAs are 19- to 24-nucleotide (nt) non-coding RNA molecules that regulate the translation of target genes through RNA interference. In UVB-exposed cells, miRNAs act on crucial functions, such as apoptosis and cellular senescence. miRNA expression is significantly altered during the protective process induced by phytochemicals. Therefore, understanding changes that occur in miRNA expression profiles may help to elucidate the protective mechanisms of troxerutin. We identified 11 miRNAs that were significantly (>2-fold) upregulated and 12 that were significantly downregulated (>2-fold) following treatment of the nHDFs with troxerutin. In addition, we investigated the biological functions of these miRNAs through the prediction of miRNA targets and Gene Ontology analysis of the putative targets. Overall, our findings indicate that pre-treatment with troxerutin increases the viability of UVB-exposed nHDFs through the alteration of the miRNA expression profiles.
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2014-April
Volume 33 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cha HJ, Lee KS, Lee GT, Lee KK, Hong JT, Lee SN, Jang HH, Lee JH, Park I, Kim YR, Kim YR, et al: Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts. Int J Mol Med 33: 957-963, 2014
APA
Cha, H.J., Lee, K.S., Lee, G.T., Lee, K.K., Hong, J.T., Lee, S.N. ... Bae, S. (2014). Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts. International Journal of Molecular Medicine, 33, 957-963. https://doi.org/10.3892/ijmm.2014.1647
MLA
Cha, H. J., Lee, K. S., Lee, G. T., Lee, K. K., Hong, J. T., Lee, S. N., Jang, H. H., Lee, J. H., Park, I., Kim, Y. R., Ahn, K. J., Kwon, S. B., An, I., An, S., Bae, S."Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts". International Journal of Molecular Medicine 33.4 (2014): 957-963.
Chicago
Cha, H. J., Lee, K. S., Lee, G. T., Lee, K. K., Hong, J. T., Lee, S. N., Jang, H. H., Lee, J. H., Park, I., Kim, Y. R., Ahn, K. J., Kwon, S. B., An, I., An, S., Bae, S."Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts". International Journal of Molecular Medicine 33, no. 4 (2014): 957-963. https://doi.org/10.3892/ijmm.2014.1647