Differentially expressed microRNAs in TGFβ2-induced epithelial-mesenchymal transition in retinal pigment epithelium cells

  • Authors:
    • Xiaoyun Chen
    • Shaobi Ye
    • Wei Xiao
    • Lixia Luo
    • Yizhi Liu
  • View Affiliations

  • Published online on: March 7, 2014     https://doi.org/10.3892/ijmm.2014.1688
  • Pages: 1195-1200
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The epithelial-mesenchymal transition (EMT) of retinal pigment epithelium (RPE) cells plays a key role in proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), both of which lead to severe loss of vision. Recently, microRNAs (miRNAs) have been found to be involved in the regulation of various physiological and pathological processes, such as embryogenesis, organ development, oncogenesis and angiogenesis. However, the expression profile and function of miRNAs in the EMT of RPE cells remain to be clarified. In this study, human miRNA expression profiles were identified using microarrays and 304 miRNAs were found to be differentially expressed in TGFβ2-induced EMT in human RPE cells. Of these differentially expressed miRNAs, 185 miRNAs were downregulated and 119 miRNAs were upregulated at least 2-fold in TGFβ2 treatment samples. Similar alterations of miRNA expression were validated for 35 representative miRNAs by quantitative polymerase chain reaction analysis. Therefore, these results suggested that differentially expressed miRNAs play potential roles in TGFβ2-induced EMT in RPE cells. This is an essential step in the identification of miRNAs associated with PVR and PDR progression, and in the identification of potential therapeutic targets for these diseases.
View Figures
View References

Related Articles

Journal Cover

May-2014
Volume 33 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Ye S, Xiao W, Luo L and Liu Y: Differentially expressed microRNAs in TGFβ2-induced epithelial-mesenchymal transition in retinal pigment epithelium cells. Int J Mol Med 33: 1195-1200, 2014
APA
Chen, X., Ye, S., Xiao, W., Luo, L., & Liu, Y. (2014). Differentially expressed microRNAs in TGFβ2-induced epithelial-mesenchymal transition in retinal pigment epithelium cells. International Journal of Molecular Medicine, 33, 1195-1200. https://doi.org/10.3892/ijmm.2014.1688
MLA
Chen, X., Ye, S., Xiao, W., Luo, L., Liu, Y."Differentially expressed microRNAs in TGFβ2-induced epithelial-mesenchymal transition in retinal pigment epithelium cells". International Journal of Molecular Medicine 33.5 (2014): 1195-1200.
Chicago
Chen, X., Ye, S., Xiao, W., Luo, L., Liu, Y."Differentially expressed microRNAs in TGFβ2-induced epithelial-mesenchymal transition in retinal pigment epithelium cells". International Journal of Molecular Medicine 33, no. 5 (2014): 1195-1200. https://doi.org/10.3892/ijmm.2014.1688