Tetrandrine suppresses pro-inflammatory mediators in PMA plus A23187-induced HMC-1 cells

Kang,Ok-Hwa; An,Hyeon-Jin; Kim,Sung-Bae; Mun,Su-Hyun; Seo,Yun-Soo; Joung,Dae-Ki; Choi,Jang-Gi; Shin,Dong-Won; Kwon,Dong-Yeul

doi:10.3892/ijmm.2014.1683

Tetrandrine suppresses pro-inflammatory mediators in PMA plus A23187-induced HMC-1 cells

Authors:
- Ok-Hwa Kang
- Hyeon-Jin An
- Sung-Bae Kim
- Su-Hyun Mun
- Yun-Soo Seo
- Dae-Ki Joung
- Jang-Gi Choi
- Dong-Won Shin
- Dong-Yeul Kwon
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Affiliations: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Iksan, Jeonbuk 540-749, Republic of Korea, BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea, Department of Oriental Medicine Resources, Sunchon National University, Sunchon, Jeonnam 540-742, Republic of Korea
Published online on: March 4, 2014 https://doi.org/10.3892/ijmm.2014.1683
Pages: 1335-1340

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Abstract

Tetrandrine (TET), a bis-benzylisoquinoline alkaloid from the root of Stephania tetrandra, is known to possess antitumor activity in various malignant neoplasms. However, the precise mechanism of TET‑mediated immune modulation remains to be clarified. One of the possible mechanisms for its protective properties is by downregulation of the inflammatory responses. In the present study, the human mast cell line (HMC-1) was used to investigate this effect. TET significantly inhibited the induction of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 by phorbol 12-myristate 13-acetate (PMA) plus A23187. Moreover, TET attenuated expression of cyclooxygenase (COX)-2. In activated HMC-1 cells, the phosphorylation of extra-signal response kinase (ERK1/2) and c-jun N-terminal Kinase (JNK1/2), but not p38 mitogen-activated protein kinase, was decreased by treatment of the cells with TET. TET inhibited PMA plus A23187-induced nuclear factor (NF)-κB activation, IκB degradation and phosphorylation. Furthermore, TET suppressed the expression of TNF-α, IL-8, IL-6 and COX-2 through suppression of the ERK1/2, JNK1/2, IκBα degradation and phosphorylation, and NF-κB activation. These results indicated that TET exerted a regulatory effect on inflammatory reactions mediated by mast cells.

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