Open Access

Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study

  • Authors:
    • Wen Zhang
    • Xiaolei Zhang
    • Junqi Ling
    • Wei Liu
    • Xinchun  Zhang
    • Jinglei Ma
    • Jianmao Zheng
  • View Affiliations

  • Published online on: July 24, 2014     https://doi.org/10.3892/ijmm.2014.1862
  • Pages: 1004-1012
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Stem cells from the apical papilla (SCAP) have odontogenic potential, which plays a pivotal role in the root dentin development of permanent teeth. Human bone morphogenetic protein 2 (BMP2) is a well-known gene that participates in regulating the odontogenic differentiation of dental tissue‑derived stem cells. However, little is known regarding the effects of the BMP2 gene on the proliferation and odontogenic differentiation of SCAP. This study aimed to evaluate the odontogenic differentiation potential of lentiviral‑mediated BMP2 gene‑transfected human SCAP (SCAP/BMP2) in vitro. SCAP were isolated by enzymatic dissociation of human teeth apical papillae. The multipotential of SCAP was verified by their osteogenic and adipogenic differentiation characteristics. The phenotype of SCAP was evaluated by flow cytometry (FCM). The proliferation status of the blank vector‑transfected SCAP (SCAP/Vector) and SCAP/BMP2 was analyzed by a cell counting kit-8 (CCK‑8). Odontogenic genes, including alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) of the two groups of cells were evaluated by quantitative polymerase chain reaction (qPCR). ALP staining and alizarin red (AR) staining of the cells was performed on the 16th day after transfection. In vitro results of CCK-8, qPCR, ALP and AR staining demonstrated that: ⅰ) SCAP/BMP2 had a comparable proliferation rate to SCAP/Vector; ⅱ) SCAP/BMP2 presented significantly better potential to differentiate into odontoblasts compared to SCAP/Vector by upregulating ALP, OCN, DSPP and DMP1 genes; ⅲ) more ALP granules and mineralized deposits were formed by SCAP/BMP2 as compared to SCAP/Vector. The results suggested that lentiviral-mediated BMP2 gene transfection enhances the odontogenic differentiation capacity of human SCAP in vitro.
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October 2014
Volume 34 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang W, Zhang X, Ling J, Liu W, Zhang X, Ma J and Zheng J: Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. Int J Mol Med 34: 1004-1012, 2014
APA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., & Zheng, J. (2014). Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. International Journal of Molecular Medicine, 34, 1004-1012. https://doi.org/10.3892/ijmm.2014.1862
MLA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34.4 (2014): 1004-1012.
Chicago
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34, no. 4 (2014): 1004-1012. https://doi.org/10.3892/ijmm.2014.1862