AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells

  • Authors:
    • Xiangjun Meng
    • Cuida Meng
    • Bing Yang
    • Li Zhao
    • Xuefei Sun
    • Yun Su
    • Hongyang Liu
    • Feiyue Fan
    • Xiaodong Liu
    • Lili Jia
  • View Affiliations

  • Published online on: July 17, 2014     https://doi.org/10.3892/ijmm.2014.1857
  • Pages: 1094-1100
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Abstract

Cumulative findings have demonstrated that the dysregulation of tumor suppressor genes may be implicated in cigarette smoke-induced carcinogenesis. Activating enhancer-binding protein 2 (AP-2) is a eukaryotic transcriptional factor that plays a significant role in embryonic development and tumorigenesis. The vertebrate AP-2 family consists of AP-2α, AP-2β, AP-2γ, AP-2δ and AP-2ε. Previous studies have suggested that cigarette smoking disrupts AP-2 regulation. In the present study, we investigated the effects of cigarette smoke condensate (CSC) on AP-2α expression in human lung cancer cell lines (NCI-H1299, NCI-H446 and A549), as well as the potential mechanisms involved. Using RT-qPCR, we found that CSC decreased AP-2α expression by suppressing its transcription in human lung cancer cell lines, particularly in p53-deficient NCI-H1299 cells. Western blotting and luciferase assays were implemented and we found that the restoration of p53 expression rescued the NCI-H1299 cells from CSC-induced AP-2α loss, while the silencing of p53 resulted in increased AP-2α loss induced by CSC, suggesting an antagonizing role of p53 in the regulation of AP-2α by CSC. Our results indicate that AP-2α downregulation may be involved in smoke-induced lung carcinogenesis.
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October 2014
Volume 34 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Meng X, Meng C, Yang B, Zhao L, Sun X, Su Y, Liu H, Fan F, Liu X, Jia L, Jia L, et al: AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells. Int J Mol Med 34: 1094-1100, 2014
APA
Meng, X., Meng, C., Yang, B., Zhao, L., Sun, X., Su, Y. ... Jia, L. (2014). AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells. International Journal of Molecular Medicine, 34, 1094-1100. https://doi.org/10.3892/ijmm.2014.1857
MLA
Meng, X., Meng, C., Yang, B., Zhao, L., Sun, X., Su, Y., Liu, H., Fan, F., Liu, X., Jia, L."AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells". International Journal of Molecular Medicine 34.4 (2014): 1094-1100.
Chicago
Meng, X., Meng, C., Yang, B., Zhao, L., Sun, X., Su, Y., Liu, H., Fan, F., Liu, X., Jia, L."AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells". International Journal of Molecular Medicine 34, no. 4 (2014): 1094-1100. https://doi.org/10.3892/ijmm.2014.1857