Open Access

Adseverin knockdown inhibits osteoclastogenesis in RAW264.7 cells

Retraction in: /10.3892/ijmm.2014.1990

  • Authors:
    • Wenting Qi
    • Yan Gao
    • Jun Tian
    • Hongwei Jiang
  • View Affiliations

  • Published online on: September 22, 2014     https://doi.org/10.3892/ijmm.2014.1941
  • Pages: 1483-1491
  • Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteoclastogenesis is a complex process that is highly dependent on the dynamic regulation of the actin cytoskeleton. Adseverin (Ads), a member of the gelsolin superfamily of actin‑binding proteins, regulates actin remodeling by severing and capping actin filaments. The objective of the present study was to characterize the role of Ads during osteoclastogenesis by assessing Ads expression and using a knockdown strategy. Immunoblot analyses were used to examine Ads expression during osteoclastogenesis. A stable Ads knockdown macrophage cell line was generated using a retroviral shRNA construct. Osteoclast differentiation was morphologically examined via cell staining with osteoclast specific markers and light microscopy. The results showed that Ads expression was significantly increased in response to receptor activator of nuclear factor‑κB ligand during osteoclastogenesis, and Ads was highly expressed in mature osteoclasts. Ads‑knockdown macrophages showed major osteoclastogenesis defects, most likely caused by a pre‑osteoclast fusion defect. These results indicate that Ads deficiency in monocytes inhibits osteoclastogenesis. Thus, in future studies it could be noteworthy to investigate the function of Ads in bone marrow monocytes during osteoclastogenesis.

Retracted

Related Articles

Journal Cover

December-2014
Volume 34 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Qi W, Gao Y, Tian J and Jiang H: Adseverin knockdown inhibits osteoclastogenesis in RAW264.7 cells Retraction in /10.3892/ijmm.2014.1990. Int J Mol Med 34: 1483-1491, 2014
APA
Qi, W., Gao, Y., Tian, J., & Jiang, H. (2014). Adseverin knockdown inhibits osteoclastogenesis in RAW264.7 cells Retraction in /10.3892/ijmm.2014.1990. International Journal of Molecular Medicine, 34, 1483-1491. https://doi.org/10.3892/ijmm.2014.1941
MLA
Qi, W., Gao, Y., Tian, J., Jiang, H."Adseverin knockdown inhibits osteoclastogenesis in RAW264.7 cells Retraction in /10.3892/ijmm.2014.1990". International Journal of Molecular Medicine 34.6 (2014): 1483-1491.
Chicago
Qi, W., Gao, Y., Tian, J., Jiang, H."Adseverin knockdown inhibits osteoclastogenesis in RAW264.7 cells Retraction in /10.3892/ijmm.2014.1990". International Journal of Molecular Medicine 34, no. 6 (2014): 1483-1491. https://doi.org/10.3892/ijmm.2014.1941