miR‑96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer

  • Authors:
    • Xuan Huang
    • Wei Lv
    • Jian‑Hua Zhang
    • Da‑Lin Lu
  • View Affiliations

  • Published online on: September 19, 2014     https://doi.org/10.3892/ijmm.2014.1940
  • Pages: 1599-1605
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Abstract

microRNA‑96 (miR‑96) is known to be downregulated in pancreatic cancer. The overexpression of miR‑96 in MIA PaCa‑2 pancreatic cancer cells has been shown to inhibit cell proliferation, migration and invasion; however, the mechanisms involved have not yet been fully elucidated. Novel (nua) kinase family 1 (NUAK1) functions as an oncogene in non‑small cell lung cancer (NSCLC), melanoma, glioma, breast cancer, hepatocellular carcinoma and pancreatic cancer. In this study, firstly, we demonstrate that NUAK1 expression is specifically upregulated in pancreatic cancer and that it promotes the proliferation, migration and invasion of MIA PaCa‑2 pancreatic cancer cells. Secondly, we performed an analysis of potential microRNA (miRNA) target sites using three commonly used prediction algorithms: miRanda, TargetScan and PicTar. All three algorithms predicted that miR‑96 targets the 3' untranslated region (3' UTR) of NUAK1. Further experiments confirmed this prediction, namely that miR‑96 suppresses the expression of NUAK1 by targeting its 3' UTR. Finally, we demonstrate that the introduction of NUAK1 cDNA lacking predicted sites of the 3' UTR abrogates miR‑96 cellular function.
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December-2014
Volume 34 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Huang X, Lv W, Zhang JH and Lu DL: miR‑96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer. Int J Mol Med 34: 1599-1605, 2014
APA
Huang, X., Lv, W., Zhang, J., & Lu, D. (2014). miR‑96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer. International Journal of Molecular Medicine, 34, 1599-1605. https://doi.org/10.3892/ijmm.2014.1940
MLA
Huang, X., Lv, W., Zhang, J., Lu, D."miR‑96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer". International Journal of Molecular Medicine 34.6 (2014): 1599-1605.
Chicago
Huang, X., Lv, W., Zhang, J., Lu, D."miR‑96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer". International Journal of Molecular Medicine 34, no. 6 (2014): 1599-1605. https://doi.org/10.3892/ijmm.2014.1940