Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells

  • Authors:
    • Zeming Wang
    • Li Ding
    • Sihan Zhang
    • Tao Jiang
    • Yongmian Yang
    • Rongheng Li
  • View Affiliations

  • Published online on: September 30, 2014     https://doi.org/10.3892/ijmm.2014.1952
  • Pages: 1720-1726
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Abstract

Arthrodial cartilage degradation and subchondral bone remodeling comprise the most predominant pathological changes in osteoarthritis (OA). Moreover, accumulating evidence indicates that the abnormal expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) and receptor activator of nuclear factor kappa-B (RANK) plays a vital role in the collapse of cartilage and subchondral bone. In the present study, the effects of icariin on the expression levels of these 3 factors in interleukin (IL)‑1β‑stimulated SW1353 chondrosarcoma cells were investigated. The SW1353 chondrosarcoma cells were cultured in the presence or absence of icariin and mitogen‑activated protein kinase signaling pathway inhibitors, and were then stimulated with IL‑1β. Cell viability was assessed by MTT assay. The mRNA and protein expression of OPG, RANKL and RANK was analyzed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and ELISA, respectively. In addition, the levels of phosphorylated p38 (p‑p38) and phosphorylated extracellular signal‑regulated kinase (p-ERK)1/2 were detected by western blot analysis. The results from western blot analysis revealed that treatment with icariin decreased the levels of p‑p38 and increased the levels of p‑ERK1/2 in the IL‑1β‑stimulated SW1353 cells. In addition, treatment with icariin decreased the levels of RANK and RANKL. Furthermore, the suppressive effects of icariin on OPG and OPG/RANKL were greater than those exhibited by the p38 signaling pathway inhibitor (SB203580). The findings of the the present study suggest that icariin has therapeutic potential for use in the treatment of OA.
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December-2014
Volume 34 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang Z, Ding L, Zhang S, Jiang T, Yang Y and Li R: Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells. Int J Mol Med 34: 1720-1726, 2014
APA
Wang, Z., Ding, L., Zhang, S., Jiang, T., Yang, Y., & Li, R. (2014). Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells. International Journal of Molecular Medicine, 34, 1720-1726. https://doi.org/10.3892/ijmm.2014.1952
MLA
Wang, Z., Ding, L., Zhang, S., Jiang, T., Yang, Y., Li, R."Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells". International Journal of Molecular Medicine 34.6 (2014): 1720-1726.
Chicago
Wang, Z., Ding, L., Zhang, S., Jiang, T., Yang, Y., Li, R."Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells". International Journal of Molecular Medicine 34, no. 6 (2014): 1720-1726. https://doi.org/10.3892/ijmm.2014.1952