Open Access

Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload

  • Authors:
    • Chuntao Wu
    • Shimin Dong
    • Yongjun Li
  • View Affiliations

  • Published online on: February 18, 2015     https://doi.org/10.3892/ijmm.2015.2105
  • Pages: 893-900
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

microRNAs (miRNAs or miRs) are essential in cardiac hypertrophy and in the development of heart failure. In the present study, we aimed to determine whether the restoration of miRNA-455 (miR-455) gene expression in vivo aggravates hypertrophy, but protects against adverse cardiac remodeling induced by pressure overload. Cardiac hypertrophy was induced by left ventricular pressure overload in male mice subjected to transverse aortic constriction (TAC). The mice were randomly selected to receive a tail vein injection of either miR-455 or green fluorescent protein per animal at 1, 8, 15 and 21 days following surgery. Cardiac hypertrophy, function and remodeling were evaluated by echocardiography, catheterization, histological analysis and the examination of the expression of specific genes and cardiac apoptosis. TAC (2 weeks following surgery) resulted in significant cardiac hypertrophy, which was significantly aggravated by treatment with miR-455. However, miR-455 replacement therapy markedly reduced myocardial fibrosis and inhibited apoptosis, suggesting that this therapy can prevent maladaptive ventricular remodeling. miR-455 was also identified and validated to target calreticulin, a protein that is critical for cardiac development. The restoration of miR-455 gene expression may thus be a potential therapeutic strategy to reverse pressure-induced cardiac hypertrophy and prevent maladaptive cardiac remodeling through the regulation of miR-455 at different time points following hypertrophy.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wu C, Dong S and Li Y: Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload. Int J Mol Med 35: 893-900, 2015
APA
Wu, C., Dong, S., & Li, Y. (2015). Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload. International Journal of Molecular Medicine, 35, 893-900. https://doi.org/10.3892/ijmm.2015.2105
MLA
Wu, C., Dong, S., Li, Y."Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload". International Journal of Molecular Medicine 35.4 (2015): 893-900.
Chicago
Wu, C., Dong, S., Li, Y."Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload". International Journal of Molecular Medicine 35, no. 4 (2015): 893-900. https://doi.org/10.3892/ijmm.2015.2105