Protective effect of ixerisoside A against UVB‑induced pro‑inflammatory cytokine production in human keratinocytes

  • Authors:
    • Sung‑Bae Kim
    • Ji‑Eun Kim
    • Ok‑Hwa Kang
    • Su‑Hyun Mun
    • Yun‑Soo Seo
    • Da‑Hye Kang
    • Da‑Wun Yang
    • Shi‑Yong Ryu
    • Young‑Mi Lee
    • Dong‑Yeul Kwon
  • View Affiliations

  • Published online on: March 2, 2015     https://doi.org/10.3892/ijmm.2015.2120
  • Pages: 1411-1418
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Human skin is the first line of defense for the protection of the internal organs of the body from different stimuli. Ultraviolet B (UVB), one of the harmful radiations for skin, is widely known to induce abnormally increased cytokine release from keratinocytes leading to inflammatory skin disorders. IL‑6 and IL‑8 induce an acute‑phase response and stimulate leukocyte infiltration in the skin. Previous studies have shown that chronic exposure to UVB radiation increases cyclooxygenase‑2 (COX‑2) expression through various cell signaling pathways, resulting in skin cancer. Recent studies have shown that the activation of extracellular signal‑regulated kinase (ERK), c‑Jun NH2‑terminal kinase (JNK), and p38 MAPK is strongly correlated with acute inflammation and development of skin cancer caused by an increased expression of COX‑2. Ixerisoside A (IXA) is an active constituent of Ixeris dentata of the Compositae (Asteraceae) family. The effect of IXA on skin inflammation has yet to be elucidated. To determine the anti‑inflammatory effects of IXA, we examined its effect on UVB‑induced pro‑inflammatory cytokine production in human keratinocytes (HaCaT cells) by observing these cells in the presence or absence of IXA. In this study, pro‑inflammatory cytokine production was determined by enzyme‑linked immunosorbent assay (ELISA), reverse transcription‑polymerase chain reaction (rt‑pcr), and western blot analysis to evaluate the activation of mitogen‑activated protein kinases (MAPKs). IXA inhibited UVB‑induced production of the pro‑inflammatory cytokines IL‑6 and IL‑8 in a dose‑dependent manner. Moreover, IXA inhibited the expression of COX‑2, ERK, JNK, and p38 MAPKs, indicating that the secretion of the pro‑inflammatory cytokines IL‑6 and IL‑8, and COX‑2 expression was inhibited by blocking MAPK phosphorylation. These results indicated that IXA potentially protects against UVB‑induced skin inflammation.
View Figures
View References

Related Articles

Journal Cover

May-2015
Volume 35 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kim SB, Kim JE, Kang OH, Mun SH, Seo YS, Kang DH, Yang DW, Ryu SY, Lee YM, Kwon DY, Kwon DY, et al: Protective effect of ixerisoside A against UVB‑induced pro‑inflammatory cytokine production in human keratinocytes. Int J Mol Med 35: 1411-1418, 2015
APA
Kim, S., Kim, J., Kang, O., Mun, S., Seo, Y., Kang, D. ... Kwon, D. (2015). Protective effect of ixerisoside A against UVB‑induced pro‑inflammatory cytokine production in human keratinocytes. International Journal of Molecular Medicine, 35, 1411-1418. https://doi.org/10.3892/ijmm.2015.2120
MLA
Kim, S., Kim, J., Kang, O., Mun, S., Seo, Y., Kang, D., Yang, D., Ryu, S., Lee, Y., Kwon, D."Protective effect of ixerisoside A against UVB‑induced pro‑inflammatory cytokine production in human keratinocytes". International Journal of Molecular Medicine 35.5 (2015): 1411-1418.
Chicago
Kim, S., Kim, J., Kang, O., Mun, S., Seo, Y., Kang, D., Yang, D., Ryu, S., Lee, Y., Kwon, D."Protective effect of ixerisoside A against UVB‑induced pro‑inflammatory cytokine production in human keratinocytes". International Journal of Molecular Medicine 35, no. 5 (2015): 1411-1418. https://doi.org/10.3892/ijmm.2015.2120