Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells

  • Authors:
    • Qiao Yu
    • Xi Chen
    • Xia Fang
    • Qiong Chen
    • Chengping Hu
  • View Affiliations

  • Published online on: March 11, 2015     https://doi.org/10.3892/ijmm.2015.2133
  • Pages: 1435-1442
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Airway mucus hypersecretion is a major pathological characteristic of chronic obstructive pulmonary disease (COPD), and cigarette smoke is highly implicated in mucus secretion and the development of COPD. Cigarette smoke reportedly induces mucin overproduction through the epidermal growth factor receptor (EGFR) in the airway epithelium; however, the underlying mechanisms responsible for the activation of EGFR remain unknown. Caveolin-1, a component protein in the cytomembrane, reportedly regulates airway inflammation and lung injury. In this study, we aimed to determine whether caveolin-1 modulates mucin hyperproduction induced by cigarette smoke. Our results revealed that cigarette smoke extract (CSE) significantly increased MUC5AC production, as well as the levels of phosphorylated EGFR (p-EGFR) and phosphorylated Akt (p-Akt) in human bronchial epithelial cells (16HBE cells), as shown by ELISA, RT-PCR and western blot analysis. These effects were prevented by treatment with EGFR inhibitor (AG1478) and phosphatidylinostol-3-kinase (PI3K) inhibitor (LY294002). We also found that the overexpression of caveolin-1 enhanced the expression of MUC5AC, p-EGFR and p-Akt induced by CSE. Conversely, the downregulation of caveolin-1 by siRNA against caveolin-1 inhibited the expression of MUC5AC, p-EGFR and p-Akt. Taken together, our data suggest that caveolin-1 enhances CSE-induced MUC5AC hypersecretion through the EGFR/PI3K/Akt signaling pathway.
View Figures
View References

Related Articles

Journal Cover

May-2015
Volume 35 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu Q, Chen X, Fang X, Chen Q and Hu C: Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells. Int J Mol Med 35: 1435-1442, 2015
APA
Yu, Q., Chen, X., Fang, X., Chen, Q., & Hu, C. (2015). Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells. International Journal of Molecular Medicine, 35, 1435-1442. https://doi.org/10.3892/ijmm.2015.2133
MLA
Yu, Q., Chen, X., Fang, X., Chen, Q., Hu, C."Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells". International Journal of Molecular Medicine 35.5 (2015): 1435-1442.
Chicago
Yu, Q., Chen, X., Fang, X., Chen, Q., Hu, C."Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells". International Journal of Molecular Medicine 35, no. 5 (2015): 1435-1442. https://doi.org/10.3892/ijmm.2015.2133