LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication

  • Authors:
    • Yangchao Dong
    • Jing Yang
    • Wei Ye
    • Yuan Wang
    • Yunbo Miao
    • Tianbing Ding
    • Chen Xiang
    • Yingfeng Lei
    • Zhikai Xu
  • View Affiliations

  • Published online on: April 7, 2015     https://doi.org/10.3892/ijmm.2015.2169
  • Pages: 1683-1689
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Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans. The DENV positive strand RNA genome contains 5' and 3' untranslated regions (UTRs) that have been shown to be required for virus replication and interaction with host cell proteins. In the present study LSm1 was identified as a host cellular protein involved in DENV RNA replication. By using two independent methodologies, we demonstrated a critical interaction between LSm1 and the 3' UTR of DENV. Furthermore, the confocal immunofluorescence analysis showed that the interaction between LSm1 and viral RNA is located in P‑body around nucleoli in the cytoplasm. LSm1 knockdown by siRNA specifically reduced the levels of viral RNA in DENV‑infected cells and infectious DENV particles in the supernatant. These results provide evidence that LSm1 binding to the DENV RNA 3' UTR positively regulates DENV RNA replication.
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June-2015
Volume 35 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Dong Y, Yang J, Ye W, Wang Y, Miao Y, Ding T, Xiang C, Lei Y and Xu Z: LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication. Int J Mol Med 35: 1683-1689, 2015
APA
Dong, Y., Yang, J., Ye, W., Wang, Y., Miao, Y., Ding, T. ... Xu, Z. (2015). LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication. International Journal of Molecular Medicine, 35, 1683-1689. https://doi.org/10.3892/ijmm.2015.2169
MLA
Dong, Y., Yang, J., Ye, W., Wang, Y., Miao, Y., Ding, T., Xiang, C., Lei, Y., Xu, Z."LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication". International Journal of Molecular Medicine 35.6 (2015): 1683-1689.
Chicago
Dong, Y., Yang, J., Ye, W., Wang, Y., Miao, Y., Ding, T., Xiang, C., Lei, Y., Xu, Z."LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication". International Journal of Molecular Medicine 35, no. 6 (2015): 1683-1689. https://doi.org/10.3892/ijmm.2015.2169