Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension

  • Authors:
    • Yongliang Jiang
    • Jing Wang
    • Hua Tian
    • Guang Li
    • Hao Zhu
    • Lei Liu
    • Ruicheng Hu
    • Aiguo Dai
  • View Affiliations

  • Published online on: May 14, 2015     https://doi.org/10.3892/ijmm.2015.2209
  • Pages: 271-281
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Abstract

Pulmonary hypertension (PH) develops in 30-70% of chronic obstructive pulmonary disease patients and increases morbidity and mortality. The present study aimed to investigate the regulation of small ubiquitin‑related modifier‑1 (SUMO‑1) expression in response to hypoxia. The experiments were carried out in vitro in rat pulmonary arterial smooth muscle cells (PASMCs) and in vivo using a rat hypoxic PH (HPH) model. A significant increase in SUMO‑1 mRNA and protein levels was observed following hypoxic stimulation in vivo and in vitro. SUMO‑1 is known to interact with various transcription factors, including hypoxia‑inducible factor‑1α (HIF‑1α) in vitro. Notably, the expression of HIF‑1α and its target gene, vascular endothelial growth factor, was increased by hypoxia in HPH. In addition, the present data suggest that SUMO‑1 regulated HIF‑1α in response to hypoxia (gene silencing and overexpression). Finally, the co‑immunoprecipitation assays suggest a direct and specific interaction between SUMO‑1 and HIF‑1α. In conclusion, SUMO‑1 may participate in the modulation of HIF‑1α through sumoylation in HPH. However, further studies are required to confirm this.
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July-2015
Volume 36 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jiang Y, Wang J, Tian H, Li G, Zhu H, Liu L, Hu R and Dai A: Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension. Int J Mol Med 36: 271-281, 2015
APA
Jiang, Y., Wang, J., Tian, H., Li, G., Zhu, H., Liu, L. ... Dai, A. (2015). Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension. International Journal of Molecular Medicine, 36, 271-281. https://doi.org/10.3892/ijmm.2015.2209
MLA
Jiang, Y., Wang, J., Tian, H., Li, G., Zhu, H., Liu, L., Hu, R., Dai, A."Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension". International Journal of Molecular Medicine 36.1 (2015): 271-281.
Chicago
Jiang, Y., Wang, J., Tian, H., Li, G., Zhu, H., Liu, L., Hu, R., Dai, A."Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension". International Journal of Molecular Medicine 36, no. 1 (2015): 271-281. https://doi.org/10.3892/ijmm.2015.2209