Apelin-13 induces MCF-7 cell proliferation and invasion via phosphorylation of ERK1/2

  • Authors:
    • Xuewei Peng
    • Fengyu Li
    • Ping Wang
    • Shengnan Jia
    • Lili Sun
    • Hongliang Huo
  • View Affiliations

  • Published online on: June 30, 2015     https://doi.org/10.3892/ijmm.2015.2265
  • Pages: 733-738
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Abstract

Apelin-13 is extensively expressed in various tissues, particularly breast tissue. Apelin‑13 has been shown to promote tumor proliferation in various types of cancer, including hepatocellular, lung and ovarian cancer. However, the effect and molecular mechanism of apelin‑13 in breast cancer cells remains unclear. The present study investigated the effect of apelin‑13 on MCF‑7. Therefore, cell proliferation was determined by MTT and flow cytometry analysis. The results revealed that apelin‑13 markedly increased cell proliferation. Transwell assays demonstrated that apelin‑13 increased MCF‑7 cell invasion. Apelin‑13 also markedly increased the expression of cyclin D1, extracellular matrix metalloproteinase‑1 and amplified in breast cancer 1 (AIB1) in a dose‑dependent manner by polymerase chain reaction assays. To study the molecular mechanism, cell proliferation, invasion and cyclin D1 were inhibited by pre‑treatment with 10 µM of PD98059 (ERK1/2 inhibitor). Western blotting results suggested that apelin‑13 significantly enhances the expression of p‑ERK1/2 in a concentration‑dependent manner. In conclusion, the results suggest that apelin‑13 promoted MCF-7 cell proliferation and invasion via the ERK1/2/AIB1 signaling pathway.
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September-2015
Volume 36 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Peng X, Li F, Wang P, Jia S, Sun L and Huo H: Apelin-13 induces MCF-7 cell proliferation and invasion via phosphorylation of ERK1/2. Int J Mol Med 36: 733-738, 2015
APA
Peng, X., Li, F., Wang, P., Jia, S., Sun, L., & Huo, H. (2015). Apelin-13 induces MCF-7 cell proliferation and invasion via phosphorylation of ERK1/2. International Journal of Molecular Medicine, 36, 733-738. https://doi.org/10.3892/ijmm.2015.2265
MLA
Peng, X., Li, F., Wang, P., Jia, S., Sun, L., Huo, H."Apelin-13 induces MCF-7 cell proliferation and invasion via phosphorylation of ERK1/2". International Journal of Molecular Medicine 36.3 (2015): 733-738.
Chicago
Peng, X., Li, F., Wang, P., Jia, S., Sun, L., Huo, H."Apelin-13 induces MCF-7 cell proliferation and invasion via phosphorylation of ERK1/2". International Journal of Molecular Medicine 36, no. 3 (2015): 733-738. https://doi.org/10.3892/ijmm.2015.2265