Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling

  • Authors:
    • Xiaolin Wu
    • Wenwei Liu
    • Hong Jiang
    • Jing Chen
    • Jichun Wang
    • Rui Zhu
    • Bin Li
  • View Affiliations

  • Published online on: December 10, 2015     https://doi.org/10.3892/ijmm.2015.2429
  • Pages: 436-444
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Abstract

It is known that vascular smooth muscle cell (VSMC) proliferation and migration leads to intimal hyperplasia in cases of atherosclerosis and restenosis. In the present study, we investigated the effects of kindlin-2 on VSMC proliferation, migration and intimal hyperplasia, and the underlying mechanisms. The left common carotid artery of Sprague‑Dawley rats were subjected to balloon injury in order to induce intimal hyperplasia, and then transfected with kindlin-2 small interfering RNA (siRNA) lentivirus or negative control siRNA lentivirus. We noted that the degree of intimal hyperplasia 4 weeks after balloon injury was significantly reduced in arteries transfected with kindlin-2 siRNA lentivirus (P<0.05). In vitro, kindlin-2 siRNA suppressed VSMC proliferation and migration induced by Wnt3a (100 ng/ml). Western blot analyses and RT-qPCR revealed that kindlin-2 regulated Wnt/β-catenin signaling and thereby modulated the expression of β-catenin target genes, including c-myc and cyclin D1. This study demonstrated that kindlin-2 plays a critical role in VSMC proliferation, migration and intimal hyperplasia via Wnt signaling. Therefore, blocking the activity of kindlin-2 represents a novel therapeutic strategy for vascular injury.
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February-2016
Volume 37 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wu X, Liu W, Jiang H, Chen J, Wang J, Zhu R and Li B: Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling. Int J Mol Med 37: 436-444, 2016
APA
Wu, X., Liu, W., Jiang, H., Chen, J., Wang, J., Zhu, R., & Li, B. (2016). Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling. International Journal of Molecular Medicine, 37, 436-444. https://doi.org/10.3892/ijmm.2015.2429
MLA
Wu, X., Liu, W., Jiang, H., Chen, J., Wang, J., Zhu, R., Li, B."Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling". International Journal of Molecular Medicine 37.2 (2016): 436-444.
Chicago
Wu, X., Liu, W., Jiang, H., Chen, J., Wang, J., Zhu, R., Li, B."Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling". International Journal of Molecular Medicine 37, no. 2 (2016): 436-444. https://doi.org/10.3892/ijmm.2015.2429