The inhibitory effect of A20 on the inflammatory reaction of epidermal keratinocytes

  • Authors:
    • Kyung-Cheol Sohn
    • Seung Ju Back
    • Dae-Kyoung Choi
    • Jung-Min Shin
    • Sue Jeong Kim
    • Myung Im
    • Young Lee
    • Young-Joon Seo
    • Tae-Jin Yoon
    • Young Ho Lee
    • Jeung-Hoon Lee
    • Chang Deok Kim
  • View Affiliations

  • Published online on: March 3, 2016     https://doi.org/10.3892/ijmm.2016.2514
  • Pages: 1099-1104
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Abstract

A20 is a negative regulator of nuclear factor κ-light‑chain-enhancer of activated B cells (NF-κB) signaling, and has been implicated in the pathogenesis of psoriasis through genome-wide association study (GWAS). In the present study, we investigated the putative role of A20 in epidermal keratinocytes. Immunohistochemical analysis showed that A20 was expressed in all layers of the epidermis, with an increasing pattern in the upper layers. In our model of calcium-induced keratinocyte differentiation, A20 expression was increased in a time-dependent manner. To investigate whether A20 affected keratinocyte differentiation, we overexpressed A20 in cultured keratinocytes. As a result, we noted that A20 overexpression did not affect keratinocyte differentiation, suggesting that A20 is not a direct modulator of keratinocyte differentiation. Interestingly, we found that A20 levels were decreased in psoriatic lesional skin compared to non-lesional areas. To investigate whether A20 played a role in the innate immune response of keratinocytes, we overexpressed A20 and then examined poly(I:C)-induced cytokine expression. We noted that A20 significantly inhibited poly(I:C)-induced cytokine production, and this effect was related to the inhibition of NF-κB signaling. These results suggest that the downregulation of A20 increased the susceptibility of keratinocytes to external stimuli, thus contributing to the development of psoriasis.
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April-2016
Volume 37 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sohn K, Back SJ, Choi D, Shin J, Kim SJ, Im M, Lee Y, Seo Y, Yoon T, Lee YH, Lee YH, et al: The inhibitory effect of A20 on the inflammatory reaction of epidermal keratinocytes. Int J Mol Med 37: 1099-1104, 2016
APA
Sohn, K., Back, S.J., Choi, D., Shin, J., Kim, S.J., Im, M. ... Kim, C.D. (2016). The inhibitory effect of A20 on the inflammatory reaction of epidermal keratinocytes. International Journal of Molecular Medicine, 37, 1099-1104. https://doi.org/10.3892/ijmm.2016.2514
MLA
Sohn, K., Back, S. J., Choi, D., Shin, J., Kim, S. J., Im, M., Lee, Y., Seo, Y., Yoon, T., Lee, Y. H., Lee, J., Kim, C. D."The inhibitory effect of A20 on the inflammatory reaction of epidermal keratinocytes". International Journal of Molecular Medicine 37.4 (2016): 1099-1104.
Chicago
Sohn, K., Back, S. J., Choi, D., Shin, J., Kim, S. J., Im, M., Lee, Y., Seo, Y., Yoon, T., Lee, Y. H., Lee, J., Kim, C. D."The inhibitory effect of A20 on the inflammatory reaction of epidermal keratinocytes". International Journal of Molecular Medicine 37, no. 4 (2016): 1099-1104. https://doi.org/10.3892/ijmm.2016.2514