Optimization of a cationic liposome-based gene delivery system for the application of miR-145 in anticancer therapeutics

  • Authors:
    • Jin Tao
    • Wei-Feng Ding
    • Xiao-Hang Che
    • Yi-Chen Chen
    • Fang Chen
    • Xiao-Dong Chen
    • Xiao-Lei Ye
    • Su-Bin Xiong
  • View Affiliations

  • Published online on: March 17, 2016     https://doi.org/10.3892/ijmm.2016.2530
  • Pages: 1345-1354
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In order to improve the delivery efficiency of microRNA (miRNA or miR)-145, the present study examined several factors which may affect cationic liposome (CL)-based transfection, including the hydration medium used for the preparation of liposomes, the quantity of the plasmid, the molar ratio of N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTAP)/cholesterol (chol), or DOTAP/chol, and the weight ratio of DOTAP/DNA. In order to enhance the transfection efficiency, protamine was selected as a DNA-condensing agent to form liposome‑protamine‑DNA (LPD) ternary complexes. An agarose gel retardation assay was used to examine the DNA binding affinity of the CLs. Following transfection, GFP fluorescence images were captured and flow cytometry was performed to determine the transfection efficiency. Furthermore, an MTT assay was performed to determine the cytotoxicity of the liposome complexes. The final optimal conditions were as follows: 5% glucose as the hydration medium, a molar ratio of DOTAP/chol at 3:1 for the preparation of CLs, a weight ratio of DOTAP/protamine/DNA of 3:0.5:1, with 8 µg plasmid added for the preparation of the LPD complexes. In vitro, the LPD complexes exhibited an enhanced transfection efficiency and low cytotoxicity, which indicated that the presented LPD vector enhanced the transfection efficiency of the CLs. The HepG2 cells were found to have the lowest expression levels of miR‑145 out of the cell lines tested (A549, BGC-823, HepG2, HeLa, LoVo and MCF-7). Following the transient transfection of the HepG2 cells with miR‑145, the results revealed that the overexpression of miR‑145 inhibited the proliferation of the HepG2 cells and downregulated the expression of cyclin-dependent kinase 6 (CDK6), cyclinD1, c-myc, and Sp1 transcription factor (Sp1). In conclusion, in this study, we optimized a liposome‑based delivery system for the efficient delivery of miR‑145 into cancer cells. This may provide a foundation for further research into the use of miR‑145 in anticancer therapeutics.
View Figures
View References

Related Articles

Journal Cover

May-2016
Volume 37 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tao J, Ding W, Che X, Chen Y, Chen F, Chen X, Ye X and Xiong S: Optimization of a cationic liposome-based gene delivery system for the application of miR-145 in anticancer therapeutics. Int J Mol Med 37: 1345-1354, 2016
APA
Tao, J., Ding, W., Che, X., Chen, Y., Chen, F., Chen, X. ... Xiong, S. (2016). Optimization of a cationic liposome-based gene delivery system for the application of miR-145 in anticancer therapeutics. International Journal of Molecular Medicine, 37, 1345-1354. https://doi.org/10.3892/ijmm.2016.2530
MLA
Tao, J., Ding, W., Che, X., Chen, Y., Chen, F., Chen, X., Ye, X., Xiong, S."Optimization of a cationic liposome-based gene delivery system for the application of miR-145 in anticancer therapeutics". International Journal of Molecular Medicine 37.5 (2016): 1345-1354.
Chicago
Tao, J., Ding, W., Che, X., Chen, Y., Chen, F., Chen, X., Ye, X., Xiong, S."Optimization of a cationic liposome-based gene delivery system for the application of miR-145 in anticancer therapeutics". International Journal of Molecular Medicine 37, no. 5 (2016): 1345-1354. https://doi.org/10.3892/ijmm.2016.2530