Open Access

Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway

  • Authors:
    • Yanyan Jia
    • Jinyi Zhao
    • Meiyou Liu
    • Bingling Li
    • Ying Song
    • Yuwen Li
    • Aidong Wen
    • Lei Shi
  • View Affiliations

  • Published online on: May 31, 2016     https://doi.org/10.3892/ijmm.2016.2616
  • Pages: 210-216
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Renal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality as there is currently no available effective therapeutic strategy with which to treat this injury. Thus, the aim of this study was to investigate the potential protective effects of brazilin, a major active component of the Chinese medicine Caesalpinia sappan L., against renal I/R injury in vitro and in vivo. Rats were subjected to removal of the right kidney and I/R injury to the left kidney (ischemia for 45 min followed by reperfusion for 24 h). Treatment with brazilin (30 mg/kg, administered intravenously at 30 min prior to ischemia) led to the reversal of I/R-induced changes in serum creatinine (Scr) and blood urea nitrogen (BUN) levels, and also attenuated the histopathological damage induced by I/R. Furthermore, TUNEL assay revealed that brazilin reduced cell necrosis, and significantly decreased the expression of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in renal tissue. Moreover, HK-2 cells were used in order to elucidate the mechanisms responsible for the protective effects of brazilin. The levels of phosphorylated IκBα and the nuclear translocation of nuclear factor-κB (NF-κB) were all evidently decreased by brazilin. These findings suggested that pre-treatment with brazilin protects against I/R-induced renal damage and suppresses the inflammatory response by inhibiting the activation of the NF-κB signaling pathway.
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July-2016
Volume 38 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jia Y, Zhao J, Liu M, Li B, Song Y, Li Y, Wen A and Shi L: Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. Int J Mol Med 38: 210-216, 2016
APA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y. ... Shi, L. (2016). Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. International Journal of Molecular Medicine, 38, 210-216. https://doi.org/10.3892/ijmm.2016.2616
MLA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38.1 (2016): 210-216.
Chicago
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38, no. 1 (2016): 210-216. https://doi.org/10.3892/ijmm.2016.2616