Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling

  • Authors:
    • Yuxian Yan
    • Haoyang Sun
    • Yuanwei Gong
    • Zhixiong Yan
    • Xizheng Zhang
    • Yong Guo
    • Yang Wang
  • View Affiliations

  • Published online on: June 10, 2016     https://doi.org/10.3892/ijmm.2016.2636
  • Pages: 594-600
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

As integrins are mechanoresponsive, there exists an intimate relationship between integrins and mechanical strain. Integrin-β1 mediates the impact of mechanical strain on bone. Mechanical strain induces bone formation through the activation of β-catenin pathways, which suggests that integrin-β1 mediates β-catenin signaling in osteoblasts in response to mechanical strain. In the present study, we examined the role of integrin-β1 in Wnt/β-catenin signal transduction in mechanically strained osteoblasts. MC3T3-E1 osteoblastic cells were transfected with integrin-β1 small interfering RNA (si-Itgβ1), and exposed to mechanical tensile strain of 2,500 microstrain (µε) using a four-point bending device. The mechanical strain enhanced the mRNA expression of integrin-β1, the protein levels of phosphorylated (p-) glycogen synthase kinase-3β (GSK‑3β) and β-catenin, simultaneously increased the mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), the protein levels of bone morphogenetic protein (BMP)-2 and -4 and enhanced the alkaline phosphatase (ALP) activity of the ME3T3-E1 cells. The elevations were inhibited by si-Itgβ1. Additionally, the mechanical strain induced the nuclear translocation of β-catenin into the nucleus, which was also inhibited by si-Itgβ1. These findings indicated that mechanical strain promoted osteoblastic differentiation through integrin‑β1‑mediated β-catenin signaling.
View Figures
View References

Related Articles

Journal Cover

August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yan Y, Sun H, Gong Y, Yan Z, Zhang X, Guo Y and Wang Y: Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling. Int J Mol Med 38: 594-600, 2016
APA
Yan, Y., Sun, H., Gong, Y., Yan, Z., Zhang, X., Guo, Y., & Wang, Y. (2016). Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling. International Journal of Molecular Medicine, 38, 594-600. https://doi.org/10.3892/ijmm.2016.2636
MLA
Yan, Y., Sun, H., Gong, Y., Yan, Z., Zhang, X., Guo, Y., Wang, Y."Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling". International Journal of Molecular Medicine 38.2 (2016): 594-600.
Chicago
Yan, Y., Sun, H., Gong, Y., Yan, Z., Zhang, X., Guo, Y., Wang, Y."Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling". International Journal of Molecular Medicine 38, no. 2 (2016): 594-600. https://doi.org/10.3892/ijmm.2016.2636