Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix

  • Authors:
    • You Hee Choi
    • Geum Soog Kim
    • Jae Ho Choi
    • Sun Woo Jin
    • Hyung Gyun Kim
    • Younho Han
    • Dae Young Lee
    • Soo Im Choi
    • Seung Yu Kim
    • Young Sup Ahn
    • Kwang Youl Lee
    • Hye Gwang Jeong
  • View Affiliations

  • Published online on: June 24, 2016     https://doi.org/10.3892/ijmm.2016.2655
  • Pages: 610-618
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Abstract

Bone remodeling and homeostasis are largely the result of the coordinated action of osteoblasts and osteoclasts. Osteoblasts are responsible for bone formation. The differentiation of osteoblasts is regulated by the transcription factors, Runx2 and Osterix. Natural products of plant origin are still a major part of traditional medicinal systems in Korea. The root of Lithospermum erythrorhizon Sieb. et Zucc. (LR), the purple gromwell, is an herbal medicine used for inflammatory and infectious diseases. LR is an anti-inflammatory and exerts anticancer effects by inducing the apoptosis of cancer cells. However, the precise molecular signaling mechanisms of osteoblastogenesis as regards LR and osteoblast transcription are not yet known. In this study, we investigated the effects of ethanol (EtOH) extract of LR (LES) on the osteoblast differentiation of C2C12 myoblasts induced by bone morphogenetic protein 4 (BMP4) and the potential involvement of Runx2 and Osterix in these effects. We found that the LES exhibited an ability to induce osteoblast differentiation. LES increased the expression of the osteoblast marker, alkaline phosphatase (ALP), as well as its activity, as shown by ALP staining and ALP activity assay. LES also increased mineralization, as shown by Alizarin Red S staining. Treatment with LES increased the protein levels (as shown by immunoblotting), as well as the transcriptional activity of Runx2 and Osterix and enhanced osteogenic activity. These results suggest that LES modulates osteoblast differentiation at least in part through Runx2 and Osterix.
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August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Choi YH, Kim GS, Choi JH, Jin SW, Kim HG, Han Y, Lee DY, Choi SI, Kim SY, Ahn YS, Ahn YS, et al: Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix. Int J Mol Med 38: 610-618, 2016
APA
Choi, Y.H., Kim, G.S., Choi, J.H., Jin, S.W., Kim, H.G., Han, Y. ... Jeong, H.G. (2016). Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix. International Journal of Molecular Medicine, 38, 610-618. https://doi.org/10.3892/ijmm.2016.2655
MLA
Choi, Y. H., Kim, G. S., Choi, J. H., Jin, S. W., Kim, H. G., Han, Y., Lee, D. Y., Choi, S. I., Kim, S. Y., Ahn, Y. S., Lee, K. Y., Jeong, H. G."Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix". International Journal of Molecular Medicine 38.2 (2016): 610-618.
Chicago
Choi, Y. H., Kim, G. S., Choi, J. H., Jin, S. W., Kim, H. G., Han, Y., Lee, D. Y., Choi, S. I., Kim, S. Y., Ahn, Y. S., Lee, K. Y., Jeong, H. G."Ethanol extract of Lithospermum erythrorhizon Sieb. et Zucc. promotes osteoblastogenesis through the regulation of Runx2 and Osterix". International Journal of Molecular Medicine 38, no. 2 (2016): 610-618. https://doi.org/10.3892/ijmm.2016.2655