Visfatin attenuates the ox-LDL-induced senescence of endothelial progenitor cells by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway

  • Authors:
    • Guang-Feng Ming
    • Yong-Jun Tang
    • Kai Hu
    • Yao Chen
    • Wei-Hua Huang
    • Jian Xiao
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  • Published online on: June 9, 2016     https://doi.org/10.3892/ijmm.2016.2633
  • Pages: 643-649
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Abstract

Endothelial progenitor cells (EPCs) play an important role in aging-associated senescence, thereby potentially contributing to vascular pathologies. Visfatin, identified as a new adipocytokine, is closely associated with the senescence of human cells. However, the effects of visfatin on the oxidized low-density lipoprotein (ox-LDL)-induced senescence of EPCs has not yet been explored, to the best of our knowledge. For this purpose, in the present study, we examined the effects of visfatin in ox-LDL-stimulated EPCs as well as the underlying mechanism responsible for these effects. We found that visfatin attenuated the ox-LDL-induced senescence of EPCs by repressing β-galactosidase expression and recovering telomerase activity. Western blot analysis confirmed that visfatin induced a dose-dependent increase in sirtuin 1 (SIRT1) expression in EPCs and ox-LDL exposure decreased SIRT1 expression. Silencing SIRT1 abolished the inhibition of EPC senescence and the suppression of p53 expression induced by visfatin. Moreover, visfatin attenuated the inhibition of phosphorylation of Akt, phosphoinositide-3-kinase (PI3K) and extracellular signal–regulated kinase (ERK) induced by ox-LDL. Taken together, these findings suggest that the treatment of EPCs with visfatin markedly attenuates the ox-LDL-induced senescence of EPCs by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway.
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August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ming G, Tang Y, Hu K, Chen Y, Huang W and Xiao J: Visfatin attenuates the ox-LDL-induced senescence of endothelial progenitor cells by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway. Int J Mol Med 38: 643-649, 2016
APA
Ming, G., Tang, Y., Hu, K., Chen, Y., Huang, W., & Xiao, J. (2016). Visfatin attenuates the ox-LDL-induced senescence of endothelial progenitor cells by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway. International Journal of Molecular Medicine, 38, 643-649. https://doi.org/10.3892/ijmm.2016.2633
MLA
Ming, G., Tang, Y., Hu, K., Chen, Y., Huang, W., Xiao, J."Visfatin attenuates the ox-LDL-induced senescence of endothelial progenitor cells by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway". International Journal of Molecular Medicine 38.2 (2016): 643-649.
Chicago
Ming, G., Tang, Y., Hu, K., Chen, Y., Huang, W., Xiao, J."Visfatin attenuates the ox-LDL-induced senescence of endothelial progenitor cells by upregulating SIRT1 expression through the PI3K/Akt/ERK pathway". International Journal of Molecular Medicine 38, no. 2 (2016): 643-649. https://doi.org/10.3892/ijmm.2016.2633