Open Access

RNAi-mediated downregulation of DNA binding protein A inhibits tumorigenesis in colorectal cancer

  • Authors:
    • Rui-Ting Liu
    • Guo-Rong Wang
    • Chang Liu
    • Jian Qiu
    • Li-Kun Yan
    • Xiao-Jun Li
    • Xiao-Qiang Wang
  • View Affiliations

  • Published online on: July 4, 2016     https://doi.org/10.3892/ijmm.2016.2662
  • Pages: 703-712
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

DNA binding protein A (dbpA) belongs to the Y-box binding protein family and has been reported to play an important role in carcinogenesis. Our previous study demonstrated that the knockdown of dbpA in gastric cancer cells inhibited cell proliferation by modulating the cell cycle. However, the role of dbpA in human colorectal cancer (CRC) remains unclear. In this study, immunohistochemical (IHC) staining and clinicopathological parameter analysis were employed to detect dbpA expression in 44 paired CRC samples and 7 CRC cell lines. Lentivirus-mediated short hairpin RNA (shRNA) was used to silence dbpA, and the effects of dbpA knockdown on cell proliferation were determined by MTT assay, colony formation assay and flow cytometry. Furthermore, a xenograft model was established to observe tumor growth in vivo. Functional analysis indicated that dbpA was overexpressed in the CRC tissues and cell lines, and a high dbpA expression was associated with the depth of invasion (p<0.001), the degree of differentiation (p<0.001), lymphatic metastasis (p<0.001) and vessel invasion (p<0.001). The suppression of dbpA expression resulted in decreased cell proliferation in vitro and tumor growth in vivo, and it induced cell cycle arrest and promoted the apoptosis of the CRC cells. As a whole, our findings illustrate the crucial role of dbpA in colorectal tumorigenesis. Thus, dbpA may be used as a novel and potent therapeutic target in CRC.
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September-2016
Volume 38 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Liu R, Wang G, Liu C, Qiu J, Yan L, Li X and Wang X: RNAi-mediated downregulation of DNA binding protein A inhibits tumorigenesis in colorectal cancer. Int J Mol Med 38: 703-712, 2016
APA
Liu, R., Wang, G., Liu, C., Qiu, J., Yan, L., Li, X., & Wang, X. (2016). RNAi-mediated downregulation of DNA binding protein A inhibits tumorigenesis in colorectal cancer. International Journal of Molecular Medicine, 38, 703-712. https://doi.org/10.3892/ijmm.2016.2662
MLA
Liu, R., Wang, G., Liu, C., Qiu, J., Yan, L., Li, X., Wang, X."RNAi-mediated downregulation of DNA binding protein A inhibits tumorigenesis in colorectal cancer". International Journal of Molecular Medicine 38.3 (2016): 703-712.
Chicago
Liu, R., Wang, G., Liu, C., Qiu, J., Yan, L., Li, X., Wang, X."RNAi-mediated downregulation of DNA binding protein A inhibits tumorigenesis in colorectal cancer". International Journal of Molecular Medicine 38, no. 3 (2016): 703-712. https://doi.org/10.3892/ijmm.2016.2662