Open Access

Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing

  • Authors:
    • Congcong Shen
    • Linlin Sun
    • Ningwen Zhu
    • Fazhi Qi
  • View Affiliations

  • Published online on: March 7, 2017     https://doi.org/10.3892/ijmm.2017.2911
  • Pages: 949-959
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The commercial use of epidermal growth factor (EGF) is extensive and has been shown to be effective for skin wound healing in clinical practice. There is evidence to indicate that the topical administration of EGF significantly accelerates re-epithelialization by promoting keratinocyte mitogenesis and migration following acute injury; however, the mechanisms involved remain to be elucidated. Thus, in this study, we focused on Kindlin-1, a four-point-one, ezrin, radixin, moesin (FERM)-domain-containing adaptor protein, and report its contribution to EGF-induced re-epithelialization in skin wound healing. In tissue samples, the expression of Kindlin-1 was induced upon EGF treatment compared to that in the natural healing group. In immortalized human keratinocytes (HaCaT cells), we further proved that Kindlin-1 was necessary for mediating EGF-induced activation signals, including integrin β1 activation, focal adhesion kinase (FAK) phosphorylation and actin re-organization, which finally led to enhanced cell proliferation and migration. These results indicate that Kindlin-1 is essential in EGF-induced re-epithelialization in skin wound healing and provide additional rationale for the clinical application of EGF in the treatment of acute wounds.
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April-2017
Volume 39 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shen C, Sun L, Zhu N and Qi F: Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing. Int J Mol Med 39: 949-959, 2017
APA
Shen, C., Sun, L., Zhu, N., & Qi, F. (2017). Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing. International Journal of Molecular Medicine, 39, 949-959. https://doi.org/10.3892/ijmm.2017.2911
MLA
Shen, C., Sun, L., Zhu, N., Qi, F."Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing". International Journal of Molecular Medicine 39.4 (2017): 949-959.
Chicago
Shen, C., Sun, L., Zhu, N., Qi, F."Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing". International Journal of Molecular Medicine 39, no. 4 (2017): 949-959. https://doi.org/10.3892/ijmm.2017.2911