SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells

  • Authors:
    • Chun-Yan Xu
    • Shi-Quan Liu
    • Meng-Bin Qin
    • Chun-Feng Zhuge
    • Lin Qin
    • Nan Qin
    • Ming-Yu Lai
    • Jie-An Huang
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  • Published online on: March 17, 2017     https://doi.org/10.3892/ijmm.2017.2921
  • Pages: 1277-1284
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Abstract

Sphingosine kinase 1 (SphK1) plays an important role in colorectal carcinoma metastasis. However, whether SphK1 modulates epithelial-mesenchymal transition (EMT)-related marker expression and the underlying mechanisms remain unclear. In this study, in order to clarify this issue, we used various colorectal cancer (CRC) cell lines, Caco2, HT29, RKO and HCT116. Each of the cell lines was divided into 3 groups as follows: the control group, SKI-Ⅱ (SphK1 inhibitor) group and PF-562271 [focal adhesion kinase (FAK) inhibitor] group. The migratory ability of the cells was examined by Transwell chamber assay. The mRNA and protein expression levels of SphK1, FAK (p-FAK), Slug, vimentin, N-cadherin and E-cadherin were detected by PCR and western blot analysis, respectively. The results revealed that the suppression of SphK1 reduced the cell migratory ability, and decreased the expression of Slug, vimentin and N-cadherin; however, the expression of E-cadherin was increased. Moreover, the inhibition of SphK1 reduced the expression of p-FAK. The inhibition of FAK (p-FAK) also decreased the cell migratory ability, and decreased the expression of Slug, vimentin and N-cadherin, whereas the expression of E-cadherin was increased. Thus, our data suggest that SphK1 modulates the expression of EMT-related markers and cell migration by regulating the expression of p-FAK in CRC cells. Thus, SphK1 may play a functional role in mediating the EMT process in CRC.
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May-2017
Volume 39 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xu C, Liu S, Qin M, Zhuge C, Qin L, Qin N, Lai M and Huang J: SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells. Int J Mol Med 39: 1277-1284, 2017
APA
Xu, C., Liu, S., Qin, M., Zhuge, C., Qin, L., Qin, N. ... Huang, J. (2017). SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells. International Journal of Molecular Medicine, 39, 1277-1284. https://doi.org/10.3892/ijmm.2017.2921
MLA
Xu, C., Liu, S., Qin, M., Zhuge, C., Qin, L., Qin, N., Lai, M., Huang, J."SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells". International Journal of Molecular Medicine 39.5 (2017): 1277-1284.
Chicago
Xu, C., Liu, S., Qin, M., Zhuge, C., Qin, L., Qin, N., Lai, M., Huang, J."SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells". International Journal of Molecular Medicine 39, no. 5 (2017): 1277-1284. https://doi.org/10.3892/ijmm.2017.2921