Open Access

Time-dependent differential expression of long non-coding RNAs following peripheral nerve injury

  • Authors:
    • Bin Pan
    • Heng-Xing Zhou
    • Yi Liu
    • Jia-Yin Yan
    • Yao Wang
    • Xue Yao
    • Yan-Qiu Deng
    • Shu-Yi Chen
    • Lu Lu
    • Zhi-Jian Wei
    • Xiao-Hong Kong
    • Shi-Qing Feng
  • View Affiliations

  • Published online on: April 21, 2017     https://doi.org/10.3892/ijmm.2017.2963
  • Pages: 1381-1392
  • Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non-coding RNAs (lncRNAs) are widely accepted as key players in various biological processes. However, the roles of lncRNA in peripheral nerve regeneration remain completely unknown. Thus, in this study, we performed microarray analysis to measure lncRNA expression in the distal segment of the sciatic nerve at 0, 3, 7 and 14 days following injury. We identified 5,354 lncRNAs that were differentially expressed: 3,788 lncRNAs were differentially expressed between days 0 and 3; 3,314 lncRNAs were differentially expressed between days 0 and 7; and 2,400 lncRNAs were differentially expressed between days 0 and 14. The results of RT-qPCR of two dysregulated lncRNAs were consistent with those of microarray analysis. Bioinformatics approaches, including lncRNA classification, gene ontology (GO) analysis and target prediction, were utilized to investigate the functions of these dysregulated lncRNAs in peripheral nerve damage. Importantly, we predicted that several lncRNA-mRNA pairs may participate in biological processes related to peripheral nerve injury. RT-qPCR was performed for the preliminary verification of three lncRNA‑mRNA pairs. The overexpression of NONMMUG014387 promoted the proliferation of mouse Schwann cells. Thus, the findings of our study may enhance our knowledge of the role of lncRNAs in nerve injury.
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June-2017
Volume 39 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Pan B, Zhou H, Liu Y, Yan J, Wang Y, Yao X, Deng Y, Chen S, Lu L, Wei Z, Wei Z, et al: Time-dependent differential expression of long non-coding RNAs following peripheral nerve injury. Int J Mol Med 39: 1381-1392, 2017
APA
Pan, B., Zhou, H., Liu, Y., Yan, J., Wang, Y., Yao, X. ... Feng, S. (2017). Time-dependent differential expression of long non-coding RNAs following peripheral nerve injury. International Journal of Molecular Medicine, 39, 1381-1392. https://doi.org/10.3892/ijmm.2017.2963
MLA
Pan, B., Zhou, H., Liu, Y., Yan, J., Wang, Y., Yao, X., Deng, Y., Chen, S., Lu, L., Wei, Z., Kong, X., Feng, S."Time-dependent differential expression of long non-coding RNAs following peripheral nerve injury". International Journal of Molecular Medicine 39.6 (2017): 1381-1392.
Chicago
Pan, B., Zhou, H., Liu, Y., Yan, J., Wang, Y., Yao, X., Deng, Y., Chen, S., Lu, L., Wei, Z., Kong, X., Feng, S."Time-dependent differential expression of long non-coding RNAs following peripheral nerve injury". International Journal of Molecular Medicine 39, no. 6 (2017): 1381-1392. https://doi.org/10.3892/ijmm.2017.2963