Open Access

Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes

  • Authors:
    • Jae-Suk Choi
    • Joo-Wan Kim
    • Jeong Been Park
    • Sang Eun Pyo
    • Yong-Ki Hong
    • Sae Kwang Ku
    • Mi-Ryung Kim
  • View Affiliations

  • Published online on: April 26, 2017     https://doi.org/10.3892/ijmm.2017.2967
  • Pages: 1437-1451
  • Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Freshwater animal proteins have long been used as nutrient supplements. In this study, melanian snail (Semisulcospira libertina) protein hydrolysates (MPh) were found to exert anti-diabetic and protective effects against liver and kidney damage in mice with type II diabetes adapted to a 45% kcal high-fat diet (HFD). The hypoglycemic, hepatoprotective and nephroprotective effects of MPh were analyzed after 12 weeks of the continuous oral administration of MPh at 125, 250 and 500 mg/kg. Diabetic control mice exhibited an increase in body weight, and blood glucose and insulin levels, with a decrease in serum high-density lipoprotein (HDL) levels. In addition, an increase in the regions of steatohepatitis, hepatocyte hypertrophy, and lipid droplet deposit-related renal tubular vacuolation degenerative lesions were detected, with noticeable expansion and hyperplasia of the pancreatic islets, and an increase in glucagon- and insulin-producing cells, insulin/glucagon cell ratios in the endocrine pancreas and hepatic lipid peroxidation, as well as decreased zymogen contents. Furthermore, a deterioration of the endogenous antioxidant defense system was observed, with reduced glucose utilization related hepatic glucokinase (GK) activity and an increase in hepatic gluconeogenesis-related phosphoenolpyruvate carboxykinase (PEPCK) and glucose‑6-phosphatase (G6pase) activity. However, all of these diabetic complications were significantly inhibited by oral treatment with MPh in a dose-dependent manner. In addition, the marked dose-dependent inhibition of hepatic lipid peroxidation, the depletion of the liver endogenous antioxidant defense system, and changes in hepatic glucose-regulating enzyme activities were also observed. The results of this study suggest that MPh exerts potent anti-diabetic effects, along with the amelioration of related complications in mice with type II diabetes. The overall effects of MPh at a dose of 125 mg/kg on HFD-induced diabetes and related complications were similar or more potent than those of metformin (250 mg/kg).
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June-2017
Volume 39 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Choi J, Kim J, Park JB, Pyo SE, Hong Y, Ku SK and Kim M: Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes. Int J Mol Med 39: 1437-1451, 2017
APA
Choi, J., Kim, J., Park, J.B., Pyo, S.E., Hong, Y., Ku, S.K., & Kim, M. (2017). Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes. International Journal of Molecular Medicine, 39, 1437-1451. https://doi.org/10.3892/ijmm.2017.2967
MLA
Choi, J., Kim, J., Park, J. B., Pyo, S. E., Hong, Y., Ku, S. K., Kim, M."Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes". International Journal of Molecular Medicine 39.6 (2017): 1437-1451.
Chicago
Choi, J., Kim, J., Park, J. B., Pyo, S. E., Hong, Y., Ku, S. K., Kim, M."Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes". International Journal of Molecular Medicine 39, no. 6 (2017): 1437-1451. https://doi.org/10.3892/ijmm.2017.2967