Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism

  • Authors:
    • Zhirong Zhang
    • Weiji Yang
    • Rongbiao Ying
    • Ying Shi
    • Huifang Jiang
    • Danli Cai
    • Jing Kuang
    • Ruhui Yang
    • Lingcong Wang
  • View Affiliations

  • Published online on: April 26, 2017     https://doi.org/10.3892/ijmm.2017.2969
  • Pages: 1580-1588
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Abstract

The present study aimed to explore the influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism (APE) in rats. Our previous study found that CX3CL1/CX3CR1 was increased in APE. However, the effect of this signaling pathway on APE remains unclear. CX3CL1-shRNA adenovirus and CX3CL1-overexpression vector were constructed. Male Sprague-Dawley rats were randomly divided into 9 groups (n=10): normal group (group N), sham operation group (group Sham), sham operation + aspirin group (group ASP), model group (group M), model + ASP group (group M+A), model + shRNA group (group M+SH), sham operation + CX3CL1-overexpression vector group (group Sham+Cx3), model + ASP + shRNA group (group M+A+SH), and model + ASP + CX3CL1-overexpression vector group (group M+A+CX3). Arterial pressure detection, hematoxylin and eosin staining, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and laser confocal scanning microscopy were applied. Aspirin significantly decreased pulmonary artery pressure, improve pathological changes in the embolism, and decreased the expression of CX3CL1/CX3CR1 and CX3CL1/NF-κB. Moreover, the adenovirus-overexpression CX3CL1 vector aggravated the inflammatory changes in APE, which were improved by aspirin. However, the intervention of the adenovirus CX3CL1 vector reduced the change, while its combination with aspirin significantly improved the change. In conclusion, aspirin improved pathological changes in rats with APE via the CX3CL1/CX3CR1 signaling pathway.
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June-2017
Volume 39 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Z, Yang W, Ying R, Shi Y, Jiang H, Cai D, Kuang J, Yang R and Wang L: Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. Int J Mol Med 39: 1580-1588, 2017
APA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D. ... Wang, L. (2017). Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism. International Journal of Molecular Medicine, 39, 1580-1588. https://doi.org/10.3892/ijmm.2017.2969
MLA
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39.6 (2017): 1580-1588.
Chicago
Zhang, Z., Yang, W., Ying, R., Shi, Y., Jiang, H., Cai, D., Kuang, J., Yang, R., Wang, L."Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism". International Journal of Molecular Medicine 39, no. 6 (2017): 1580-1588. https://doi.org/10.3892/ijmm.2017.2969