Open Access

miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1

  • Authors:
    • Jing Zhu
    • Zhejun Chen
    • Jinxing Tian
    • Zehui Meng
    • Mingda Ju
    • Gencheng Wu
    • Zhanzhuang Tian
  • View Affiliations

  • Published online on: May 9, 2017     https://doi.org/10.3892/ijmm.2017.2981
  • Pages: 90-100
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exposure to trauma is a potential contributor to anxiety; however, the molecular mechanisms responsible for trauma-induced anxiety require further clarification. In this study, in an aim to explore these mechanisms, we observed the changes in the hypothalamic pituitary adrenal (HPA) axis using a radioimmunoassay and the changes in anxiety-like behavior using the open field test and elevated plus maze test in a rat model following intervention with NBI‑27914, a specific corticotropin‑releasing hormone receptor 1 (CRHR1) antagonist. CRHR1 was found to be involved in trauma‑induced anxiety. We then applied bioinformatic analysis to screen microRNAs (miRNAs or miRs) that target CRHR1, and miR‑34b was determined to negatively regulate CRHR1 mRNA in primary hypothalamic neurons. The overexpression of miR‑34b in the paraventricular nucleus (PVN) by a miRNA agomir using a drug delivery system decreased the hyperactivity of the HPA axis and anxiety‑like behavior. Overall, the involvement of the HPA axis in trauma‑induced anxiety was demonstrated, and trauma-induced anxiety was attenuated by decreasing the hyperactivity of the HPA axis via miR‑34b by targeting CRHR1.
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July-2017
Volume 40 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhu J, Chen Z, Tian J, Meng Z, Ju M, Wu G and Tian Z: miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. Int J Mol Med 40: 90-100, 2017
APA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., & Tian, Z. (2017). miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1. International Journal of Molecular Medicine, 40, 90-100. https://doi.org/10.3892/ijmm.2017.2981
MLA
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40.1 (2017): 90-100.
Chicago
Zhu, J., Chen, Z., Tian, J., Meng, Z., Ju, M., Wu, G., Tian, Z."miR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1". International Journal of Molecular Medicine 40, no. 1 (2017): 90-100. https://doi.org/10.3892/ijmm.2017.2981