Open Access

Role of metadherin in estrogen-regulated gene expression

  • Authors:
    • Yujun Li
    • Jesus Gonzalez Bosquet
    • Shujie Yang
    • Kristina W. Thiel
    • Yuping Zhang
    • Haitao Liu
    • Kimberly K. Leslie
    • Xiangbing Meng
  • View Affiliations

  • Published online on: June 13, 2017     https://doi.org/10.3892/ijmm.2017.3020
  • Pages: 303-310
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The disruption of estrogen signaling is widely associated with the development of breast, endometrial and ovarian cancers. As a multifunctional mediator of carcinogenesis, metadherin (MTDH)/astrocyte elevated gene-1 (AEG-1) overexpression has been associated with numerous types of cancer, with reported roles in tumor initiation, proliferation, invasion, metastasis and chemoresistance. At the molecular level, MTDH has been shown to interact with proteins that drive tumorigenesis, including nuclear factor-κB (NF-κB), promyelocytic leukaemia zinc finger (PLZF), BRCA2- and CDKN1A (p21Cip1/Waf-1/mda-6)-interacting protein α (BCCIPα) and staphylococcal nuclease and tudor domain containing 1 (SND1). Through the analysis of the Cancer Genome Atlas (TCGA) datasets for estrogen receptor (ER)-positive endometrial and breast cancers, we found that over 25% of all gene expression correlated with MTDH. Using Affymetrix microarrays, we characterized the differences in gene expression between estrogen-treated parental and MTDH-deficient endometrial and breast cancer cells. We also explored a possible interaction between MTDH and ER by immunoprecipitation, and found that MTDH and ER associated in both breast and endometrial cancer cells in response to estrogen. Reciprocal co-immunoprecipitation analysis demonstrated that acute estrogen stimulation promoted the interaction of MTDH with ER in the nucleus. These data, to the best of our knowledge, provide the first evidence that MTDH and ERα interact in the nucleus with estrogen treatment to regulate gene expression.
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August-2017
Volume 40 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Li Y, Gonzalez Bosquet J, Yang S, Thiel KW, Zhang Y, Liu H, Leslie KK and Meng X: Role of metadherin in estrogen-regulated gene expression. Int J Mol Med 40: 303-310, 2017
APA
Li, Y., Gonzalez Bosquet, J., Yang, S., Thiel, K.W., Zhang, Y., Liu, H. ... Meng, X. (2017). Role of metadherin in estrogen-regulated gene expression. International Journal of Molecular Medicine, 40, 303-310. https://doi.org/10.3892/ijmm.2017.3020
MLA
Li, Y., Gonzalez Bosquet, J., Yang, S., Thiel, K. W., Zhang, Y., Liu, H., Leslie, K. K., Meng, X."Role of metadherin in estrogen-regulated gene expression". International Journal of Molecular Medicine 40.2 (2017): 303-310.
Chicago
Li, Y., Gonzalez Bosquet, J., Yang, S., Thiel, K. W., Zhang, Y., Liu, H., Leslie, K. K., Meng, X."Role of metadherin in estrogen-regulated gene expression". International Journal of Molecular Medicine 40, no. 2 (2017): 303-310. https://doi.org/10.3892/ijmm.2017.3020